Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation.
AuthorsBaez MV, Luchelli L, Maschi D, Habif M, Pascual M, Thomas MG, Boccaccio GL,
JournalJ Cell Biol
PubMed ID22201125
'Mammalian Smaug1/Samd4A is a translational repressor. Here we show that Smaug1 forms mRNA-silencing foci located at postsynapses of hippocampal neurons. These structures, which we have named S-foci, are distinct from P-bodies, stress granules, or other neuronal RNA granules hitherto described, and are the first described mRNA-silencing foci specific to neurons. ... More
Mammalian Smaug is a translational repressor that forms cytoplasmic foci similar to stress granules.
AuthorsBaez MV, Boccaccio GL,
JournalJ Biol Chem
PubMed ID16221671
'Cytoplasmic events depending on RNA-binding proteins contribute to the fine-tuning of gene expression. Sterile alpha motif-containing RNA-binding proteins constitute a novel family of post-transcriptional regulators that recognize a specific RNA sequence motif known as Smaug recognition element (SRE). The Drosophila member of this family, dSmaug, triggers the translational repression and ... More
Dynein and kinesin regulate stress-granule and P-body dynamics.
AuthorsLoschi M, Leishman CC, Berardone N, Boccaccio GL,
JournalJ Cell Sci
PubMed ID19825938
Stress granules (SGs) and P-bodies (PBs) are related cytoplasmic structures harboring silenced mRNAs. SGs assemble transiently upon cellular stress, whereas PBs are constitutive and are further induced by stress. Both foci are highly dynamic, with messenger ribonucleoproteins (mRNPs) and proteins rapidly shuttling in and out. Here, we show that impairment ... More
Mammalian Staufen 1 is recruited to stress granules and impairs their assembly.
Stress granules are cytoplasmic mRNA-silencing foci that form transiently during the stress response. Stress granules harbor abortive translation initiation complexes and are in dynamic equilibrium with translating polysomes. Mammalian Staufen 1 (Stau1) is a ubiquitous double-stranded RNA-binding protein associated with polysomes. Here, we show that Stau1 is recruited to stress ... More
Staufen recruitment into stress granules does not affect early mRNA transport in oligodendrocytes.
Staufen is a conserved double-stranded RNA-binding protein required for mRNA localization in Drosophila oocytes and embryos. The mammalian homologues Staufen 1 and Staufen 2 have been implicated in dendritic RNA targeting in neurons. Here we show that in rodent oligodendrocytes, these two proteins are present in two independent sets of ... More