Combined confocal and wide-field high-resolution cytometry of fluorescent in situ hybridization-stained cells.
AuthorsKozubek M, Kozubek S, Lukásová E, Bártová E, Skalníková M, Matula P, Matula P, Jirsová P, Cafourková A, Koutná I
JournalCytometry
PubMed ID11598941
BACKGROUND: The recently developed technique of high-resolution cytometry (HRCM) enables automated acquisition and analysis of fluorescent in situ hybridization (FISH)-stained cell nuclei using conventional wide-field fluorescence microscopy. The method has now been extended to confocal imaging and offers the opportunity to combine the advantages of confocal and wide-field modes. METHODS: ... More
Which Elements to Build Co-localization Workflows? From Metrology to Analysis.
AuthorsMascalchi P, Cordelières FP
JournalMethods Mol Biol
PubMed ID31432481
Co-localization analysis is one of the main interests of users entering a facility with slides in hands and nice analysis perspectives in mind. While being available through most, if not all, analysis software, co-localization tools are mainly perceived as black boxes, fed with images, that will, hopefully, return (the expected) ... More