Phire 组织直接 PCR 预混液
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Phire 组织直接 PCR 预混液
Thermo Scientific™

Phire 组织直接 PCR 预混液

Thermo Scientific Phire 组织直接PCR 预混液经开发用于直接从小鼠、人、鱼、鸟和昆虫获取的各种组织中扩增 DNA。含有了解更多信息
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货号反应次数
F170S250 次反应
F170L1250 次反应
货号 F170S
价格(CNY)
1,435.00
飞享价
Ends: 31-Dec-2025
2,048.00
共减 613.00 (30%)
Each
添加至购物车
反应次数:
250 次反应
请求批量或定制报价
价格(CNY)
1,435.00
飞享价
Ends: 31-Dec-2025
2,048.00
共减 613.00 (30%)
Each
添加至购物车
Thermo Scientific Phire 组织直接PCR 预混液经开发用于直接从小鼠、人、鱼、鸟和昆虫获取的各种组织中扩增 DNA。含有 Phire 热启动 II DNA 聚合酶的预混液是特别配制的,用于在不同动物组织来源的抑制剂如胶原蛋白、黑色素和真黑色素(毛发、皮肤)或肌红蛋白(肌肉)存在的情况下进行 PCR。该试剂盒还包括 Thermo Scientific DNARelease 添加剂,可用于改善从困难组织中DNA的释放。

主要特点

• 直接 PCR—将样品直接加入到 PCR 反应中,因此不需要耗时且昂贵的 DNA 纯化步骤
• 稀释&可用的储存方案—允许从一个微小样品中进行数十次 PCR 反应,并允许重新测试
• 特别设计的 Phire DNA 聚合酶—需要的 PCR 实验方案时间非常短
• 带有预混凝胶上样染料的预混液—将交叉污染的可能性降至最低,减少了样品处理,并允许直接进行凝胶上样

应用

• 基因分型
• 转基因检测
• 敲除分析
• 测序

Phire DNA 聚合酶的最佳退火温度可能与基于 Taq 的聚合酶显著不同。

为了获得最佳结果,请使用我们的 Tm 计算器准确计算 Tm。
仅供科研使用。不可用于诊断程序。
规格
热启动内置热启动
包括标签或染料
反应次数250 次反应
突出端平末端
聚合酶Phire 热启动 II DNA 聚合酶
产品类型直接 PCR 预混液
数量250 × 20 μL 反应
反应形式SuperMix 或预混液
样品类型组织
运输条件湿冰或干冰
原始材料DNA
最大浓度2X
适用于(应用)基因分型, Hot-start PCR
高 GC PCR 扩增效果
PCR 方法直接 PCR
反应速度快速
Unit SizeEach
内容与储存
包含:
• 2 x 1.25 mL Phir 组织直接 PCR 预混液 (2X)
• 5 mL 稀释液
• 3 x 100 μL DNARelease 添加剂
• 40 μL 对照引物混合物 (各25 μM )
• 2 x 1.25 mL 无核酸酶水
• 50 μg O'GeneRuler Express DNA 分子量标准(足够用于100次应用)

-20 °C 储存。

常见问题解答 (FAQ)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

How are samples, stored at different conditions, compatible with the Phire Tissue Direct PCR Master Mix?

With the Phire Tissue Direct PCR Master Mix you can amplify DNA from differently stored tissues (fresh, frozen, FFPE) from human and a variety of animal species.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.