Protonation dynamics of the extracellular and cytoplasmic surface of bacteriorhodopsin in the purple membrane.
Authors:Nachliel E,Gutman M,Kiryati S,Dencher NA
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:8855251
The dynamics of proton binding to the extracellular and the cytoplasmic surfaces of the purple membrane were measured by laser-induced proton pulses. Purple membranes, selectively labeled by fluorescein at Lys-129 of bacteriorhodopsin, were pulsed by protons released in the aqueous bulk from excited pyranine (8-hydroxy-1,3,6-pyrenetrisulfonate) and the reaction of protons ... More