Microfluidic flow-flash: method for investigating protein dynamics.
AuthorsToepke MW, Brewer SH, Vu DM, Rector KD, Morgan JE, Gennis RB, Kenis PJ, Dyer RB
JournalAnal Chem
PubMed ID17194129
We report a new method, microfluidic flow-flash, for measuring protein reaction kinetics. The method couples a microscope imaging detection system with a microfluidic flow cell to reduce data acquisition times and sample consumption. This combination allows for the simultaneous collection of spectral and temporal information. The microfluidic flow cell design ... More
Utilization of kinetically enhanced monovalent binding affinity by immunoassays based on multivalent nanoparticle-antibody bioconjugates.
AuthorsSoukka T, Härmä H, Paukkunen J, Lövgren T
JournalAnal Chem
PubMed ID11393849
The monovalent binding affinity of high binding site density nanoparticle-antibody bioconjugates is shown to exceed the intrinsic affinity of the original, monoclonal antibody. The nanoparticle-antibody bioconjugates were prepared by covalent coupling of antibodies to long-lifetime fluorescent, europium(III) chelate nanoparticles, 107 nm in diameter. Experiments were carried out in standard microtitration ... More