Deficiencies in collagen phagocytosis by human fibroblasts in vitro: a mechanism for fibrosis?
AuthorsMcCulloch CA, Knowles GC
JournalJ Cell Physiol
PubMed ID8491787
'Degradation of collagen by fibroblast phagocytosis is an important pathway for physiological remodelling of soft connective tissues. Perturbations of this pathway may provide a mechanism for the development of fibrotic lesions. As collagen phagocytosis may be regulated by either a change of the proportions or the activity of phagocytic cells, ... More
A distinct integrin-mediated phagocytic pathway for extracellular matrix remodeling by RPE cells.
AuthorsZhao MW, Jin ML, He S, Spee C, Ryan SJ, Hinton DR
JournalInvest Ophthalmol Vis Sci
PubMed ID10509670
'PURPOSE: To characterize the phagocytosis of extracellular matrix components by retinal pigment epithelial cells and to determine which receptors and signal transduction pathways are involved. METHODS: Fluorescent latex beads were coated with fibronectin (FN), collagen type I or IV, or thrombospondin and incubated with human retinal pigment epithelial cells for ... More
Mechanism of collagen phagocytosis by human gingival fibroblasts: importance of collagen structure in cell recognition and internalization.
AuthorsKnowles GC, McKeown M, Sodek J, McCulloch CA
JournalJ Cell Sci
PubMed ID1650378
'Phagocytosis of extracellular collagen by fibroblasts appears to be the principal pathway of collagen degradation in the physiological turnover of connective tissues. To study the mechanism of collagen phagocytosis, subconfluent gingival fibroblasts were serum-starved and incubated for up to 16 h with collagen-coated fluorescent latex beads. Internalization of beads was ... More
Loss-of-function mutations in Rab escort protein 1 (REP-1) affect intracellular transport in fibroblasts and monocytes of choroideremia patients.
'BACKGROUND: Choroideremia (CHM) is a progressive X-linked retinopathy caused by mutations in the CHM gene, which encodes Rab escort protein-1 (REP-1), an escort protein involved in the prenylation of Rabs. Under-prenylation of certain Rabs, as a result of loss of function mutations in REP-1, could affect vesicular trafficking, exocytosis and ... More
The membrane-anchored metalloproteinase regulator RECK stabilizes focal adhesions and anterior-posterior polarity in fibroblasts.
AuthorsMorioka Y, Monypenny J, Matsuzaki T, Shi S, Alexander DB, Kitayama H, Noda M,
JournalOncogene
PubMed ID19169281
Accumulating evidence indicates that Reversion-inducing cysteine-rich protein with Kazal motifs (RECK), a membrane-anchored matrix metalloproteinase regulator, plays crucial roles in mammalian development and tumor suppression. Its mechanisms of action at the single cell level, however, remain largely unknown. In mouse fibroblasts, RECK is abundant around the perinuclear region, membrane ruffles ... More
Latex beads as probes of a neural crest pathway: effects of laminin, collagen, and surface charge on bead translocation.
AuthorsBronner-Fraser M
JournalJ Cell Biol
PubMed ID6373786
In the trunk region of avian embryos, neural crest cells migrate along two pathways: dorsally just under the ectoderm, and ventrally between the neural tube and the somites. Previous work from this laboratory has shown that uncoated latex beads are able to translocate along the ventral neural crest pathway after ... More
Deregulation of collagen phagocytosis in aging human fibroblasts: effects of integrin expression and cell cycle.
AuthorsLee W, McCulloch CA
JournalExp Cell Res
PubMed ID9434634
Intracellular degradation of collagen by phagocytosis in fibroblasts is essential for physiological remodeling of the extracellular matrix in a wide variety of connective tissues but imbalances between degradation and synthesis can lead to loss of tissue collagen. As aging is associated with loss of dermal and periodontal collagen and with ... More