Use of coherent control methods through scattering biological tissue to achieve functional imaging.
AuthorsDela Cruz JM, Pastirk I, Comstock M, Lozovoy VV, Dantus M
JournalProc Natl Acad Sci U S A
PubMed ID15569924
'We test whether coherent control methods based on ultrashort-pulse phase shaping can be applied when the laser light propagates through biological tissue. Our results demonstrate experimentally that the spectral-phase properties of shaped laser pulses optimized to achieve selective two-photon excitation survive as the laser pulses propagate through tissue. This observation ... More
Detection technologies in proteome analysis.
AuthorsPatton WF
JournalJ Chromatogr B Analyt Technol Biomed Life Sci
PubMed ID12015990
'Common strategies employed for general protein detection include organic dye, silver stain, radiolabeling, reverse stain, fluorescent stain, chemiluminescent stain and mass spectrometry-based approaches. Fluorescence-based protein detection methods have recently surpassed conventional technologies such as colloidal Coomassie blue and silver staining in terms of quantitative accuracy, detection sensitivity, and compatibility with ... More
Visualizing regional myocardial blood flow in the mouse.
AuthorsKrueger MA, Huke SS, Glenny RW,
JournalCirc Res
PubMed ID23513055
The spatial distribution of blood flow in the hearts of genetically modified mice is a phenotype of interest because derangements in blood flow may precede detectable changes in organ function. However, quantifying the regional distribution of blood flow within organs of mice is challenging because of the small organ volume ... More
Effects of gestational age and cortisol treatment on ovine fetal heart function in a novel biventricular Langendorff preparation.
AuthorsFletcher AJ, Forhead AJ, Fowden AL, Ford WR, Nathanielsz PW, Giussani DA,
JournalJ Physiol
PubMed ID15513943
Structural and functional maturation of a number of fetal organs and physiological systems occurs in the immediate period prior to term, in association with the prepartum increase in fetal plasma cortisol concentration. At present, little is known about how myocardial sensitivity to adrenergic and muscarinic cholinergic stimulation changes as the ... More
Multiplexed spectral signature detection for microfluidic color-coded bioparticle flow.
Here, we report a high-speed photospectral detection technique capable of discriminating subtle variations of spectral signature among fluorescently labeled cells and microspheres flowing in a microfluidic channel. The key component used in our study is a strain-tunable nanoimprinted grating microdevice coupled with a photomultiplier tube (PMT). The microdevice permits acquisition ... More