AuthorsThompson RB, Peterson D, Mahoney W, Cramer M, Maliwal BP, Suh SW, Frederickson C, Fierke C, Herman P
JournalJ Neurosci Methods
PubMed ID12191759
'Mounting evidence indicates that zinc has multiple roles in cell biology, viz. as a part of metalloenzyme catalytic sites, as a structural component of gene regulatory proteins, and (like calcium) as a free signal ion, particularly in the cortex of the brain. While most Zn(II) in the brain is tightly ... More
Induction of the ZRC1 metal tolerance gene in zinc-limited yeast confers resistance to zinc shock.
AuthorsMacDiarmid CW, Milanick MA, Eide DJ
JournalJ Biol Chem
PubMed ID12556516
'Zinc is an essential nutrient but toxic to cells with overaccumulation. For this reason, intracellular zinc levels are tightly controlled. In the yeast Saccharomyces cerevisiae, the Zrc1 and Cot1 proteins have been implicated in the storage and detoxification of excess zinc in the vacuole. Surprisingly, transcription of ZRC1 is induced ... More
A reevaluation of neuronal zinc measurements: artifacts associated with high intracellular dye concentration.
AuthorsDineley KE, Malaiyandi LM, Reynolds IJ
JournalMol Pharmacol
PubMed ID12181438
The emergence of zinc as a potent neurotoxin has prompted the development of techniques suitable for the measurement of intracellular free zinc ([Zn(2+)](i)) in cultured cells. Accordingly, a new family of Zn(2+)-sensitive fluorophores has become available. Using ionophore-induced elevations of [Zn(2+)](i) in cultured neurons, we measured [Zn(2+)](i)-induced changes in the ... More