Visualization of transmitter release with zinc fluorescence detection at the mouse hippocampal mossy fibre synapse.
AuthorsQian J, Noebels JL
JournalJ Physiol
PubMed ID15919713
'Exocytosis of synaptic vesicle contents defines the quantal nature of neurotransmitter release. Here we developed a technique to directly assess exocytosis by measuring vesicular zinc release with the zinc-sensitive dye FluoZin-3 at the hippocampal mossy fibre (MF) synapse. Using a photodiode, we were able to clearly resolve the zinc fluorescence ... More