Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome.
AuthorsOhka S, Matsuda N, Tohyama K, Oda T, Morikawa M, Kuge S, Nomoto A
JournalJ Virol
PubMed ID15194795
'Poliovirus (PV), when injected intramuscularly into the calf, is incorporated into the sciatic nerve and causes an initial paralysis of the inoculated limb in transgenic mice carrying the human PV receptor (hPVR/CD155) gene. Here, we demonstrated by using an immunoelectron microscope that PV particles exist on vesicle structures in nerve ... More
Dystrophin is a component of the subsynaptic membrane.
AuthorsYeadon JE, Lin H, Dyer SM, Burden SJ
JournalJ Cell Biol
PubMed ID1720119
A subsynaptic protein of Mr approximately 300 kD is a major component of Torpedo electric organ postsynaptic membranes and copurifies with the AChR and the 43-kD subsynaptic protein. mAbs against this protein react with neuromuscular synapses in higher vertebrates, but not at synapses in dystrophic muscle. The Torpedo 300-kD protein ... More
Reducing cellular autofluorescence in flow cytometry: an in situ method.
Cellular autofluorescence affects the sensitivity of flow cytometric assays by interfering with detection of low level specific fluorescence. These detection limits increase with use of protocols, such as thermocycling and fluorescent in-situ hybridization (FISH), that can increase intrinsic cellular fluorescence to 5,000-20,000 fluorescein isothiocyanate (FITC) equivalents. In order to improve ... More
High-resolution FISH on super-stretched flow-sorted plant chromosomes.
AuthorsValárik M, Bartos J, Kovárová P, Kubaláková M, de Jong JH, Dolezel J
JournalPlant J
PubMed ID14996224
A novel high-resolution fluorescence in situ hybridisation (FISH) strategy, using super-stretched flow-sorted plant chromosomes as targets, is described. The technique that allows longitudinal extension of chromosomes of more than 100 times their original metaphase size is especially attractive for plant species with large chromosomes, whose pachytene chromosomes are generally too ... More
Dystrophin in a membrane skeletal network: localization and comparison to other proteins.
AuthorsDmytrenko GM, Pumplin DW, Bloch RJ
JournalJ Neurosci
PubMed ID8426227
We studied the location, relative abundance, and stability of dystrophin in clusters of ACh receptors (AChRs) isolated from primary cultures of neonatal rat myotubes. Although variable amounts of dystrophin were found at receptor clusters, dystrophin was always associated with organized, receptor-rich domains (AChR domains). Dystrophin was occasionally seen in focal ... More