Fc OxyBURST™ Green 检测试剂
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引用和文献 (14)
Invitrogen™

Fc OxyBURST™ Green 检测试剂

Fc OxyBURST™ Green 检测试剂包含共价连接至二氯二氢荧光素 (H2DCF) 的牛血清白蛋白 (BSA)，然后与纯化兔多克隆抗 BSA 抗体形成复合物。当这种免疫复合物与了解更多信息
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货号数量
F290225 次反应
货号 F2902
价格（CNY)
4,324.00
飞享价
Ends: 31-Dec-2025
5,745.00
共减 1,421.00 (25%)
Each
添加至购物车
数量:
25 次反应
价格（CNY)
4,324.00
飞享价
Ends: 31-Dec-2025
5,745.00
共减 1,421.00 (25%)
Each
添加至购物车
Fc OxyBURST™ Green 检测试剂包含共价连接至二氯二氢荧光素 (H2DCF) 的牛血清白蛋白 (BSA)，然后与纯化兔多克隆抗 BSA 抗体形成复合物。当这种免疫复合物与 Fc 受体结合时，非荧光 H2DCF 分子在吞噬细胞中内化，随后氧化为绿色荧光 2''7'-二氯荧光素。
仅供科研使用。不可用于诊断程序。
规格
最大浓度3 mg⁄ml
检测方法荧光
染料类型自由基传感器
形式溶液
产品规格管、玻片
数量25 次反应
运输条件湿冰
溶解度DMSO（二甲亚砜）
发射488⁄523
适用于（设备）荧光显微镜, 流式细胞仪
产品线OXYBURST
产品类型测定试剂
Unit SizeEach
内容与储存
包含 1 小瓶 Fc OxyBURST™ Green 检测试剂（悬浮于含叠氮化钠的 PBS 中）。 避光储存在冰箱 (2–8°C) 中。

引用和文献 (14)

引用和文献
Abstract
The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis.
Authors:Suh CI, Stull ND, Li XJ, Tian W, Price MO, Grinstein S, Yaffe MB, Atkinson S, Dinauer MC
Journal:J Exp Med
PubMed ID:16880255
'Superoxide produced by the phagocyte reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is essential for host defense. Enzyme activation requires translocation of p67(phox), p47(phox), and Rac-GTP to flavocytochrome b558 in phagocyte membranes. To examine the regulation of phagocytosis-induced superoxide production, flavocytochrome b558, p47(phox), p67(phox), and the FcgammaIIA receptor were expressed ... More
Severe impairment in early host defense against Listeria monocytogenes in mice deficient in acid sphingomyelinase.
Authors:Utermöhlen O, Karow U, Löhler J, Krönke M
Journal:J Immunol
PubMed ID:12594290
'The phagolysosomal compartment is crucial for the defense against infection with intracellular pathogens. Within this compartment, the TNF- and IFN-gamma-responsive acid sphingomyelinase (ASMase) generates the signaling molecule ceramide, resulting in the activation of proteases like cathepsin D. To investigate the possible role of ASMase as a mediator of the antibacterial ... More
Quantitative multiwell myeloid differentiation assay using dichlorodihydrofluorescein diacetate (H2DCF-DA) or dihydrorhodamine 123 (H2R123).
Authors:Trayner ID, Rayner AP, Freeman GE, Farzaneh F
Journal:J Immunol Methods
PubMed ID:7594627
'It is well established that the fluorescent probes dichlorodihydrofluorescein diacetate (H2DCF-DA) and dihydrorhodamine 123 (H2R123) can be used to detect the respiratory burst response of mature myeloid cells. We describe a simple, fast and quantitative assay for myeloid differentiation based on the oxidation of these probes, which can be performed ... More
A cytosolic calcium transient is not necessary for degranulation or oxidative burst in immune complex-stimulated neutrophils.
Authors:Seetoo KF, Schonhorn JE, Gewirtz AT, Zhou MJ, McMenamin ME, Delva L, Simons ER
Journal:J Leukoc Biol
PubMed ID:9307071
'Receptor-mediated activation of neutrophils (PMN) initiates possibly interdependent events, including a rapid transient increase in [Ca2+]i, implicated as a second messenger. To investigate whether this transient is required for eventual degranulation, PMN were incubated with an intracellular Ca2+ chelator (BAPTA), then exposed to chemotactic peptide [N-formyl-methionyl-leucyl-phenylalanine (fMLP)l with or without ... More
Fluorescent probes for living cells.
Authors:Johnson I
Journal:Histochem J
PubMed ID:10188922
'The functional characteristics of fluorescent probes used for imaging and measuring dynamic processes in living cells are reviewed. Initial consideration is given to general design requirements for delivery, targeting, detectability and fluorescence readout, and current technologies for attaining them. Discussion then proceeds to the more application-specific properties of intracellular ion ... More
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