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Citations & References (22)
Invitrogen™
FluoReporter™ lacZ/Galactosidase Quantitation Kit
The FluoReporter™ lacZ/Galactosidase Quantitation Kit includes the β-galactosidase substrate, 3-carboxyumbelliferyl β-D-galactopyranoside that produces the blue-fluorescent hydrolysis product, 7-hydroxycoumarin-3-carboxlic acid.Read more
| Catalog Number | Quantity |
|---|---|
| F2905 | 1 Kit |
Catalog number F2905
Price (CNY)
5,108.00
Each
Quantity:
1 Kit
Price (CNY)
5,108.00
Each
The FluoReporter™ lacZ/Galactosidase Quantitation Kit includes the β-galactosidase substrate, 3-carboxyumbelliferyl β-D-galactopyranoside that produces the blue-fluorescent hydrolysis product, 7-hydroxycoumarin-3-carboxlic acid.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assayβ-Gal (lacZ) Assay
Detection MethodFluorescence
Product LineFluoReporter
Product TypeBeta-Galactosidase Reporter Gene Assay System
Quantity1 Kit
Shipping ConditionRoom Temperature
SubstrateCUG
Substrate PropertiesChemical Substrate
Substrate TypeBeta-Gal Substrate
Target EnzymeBeta-Galactosidase
FormatKit
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (22)
Citations & References
Abstract
Substrate-labeled fluorescent immunoassay for phenobarbital.
Journal:Ther Drug Monit
PubMed ID:7013165
An assay for the anticonvulsant drug phenobarbital (PB) has been developed that is based on the principles of the substrate-labeled fluorescent immunoassay. A fluorogenic enzyme substrate, galactosyl umbelliferone, was covalently linked to a derivative of PB. The labeled drug, galactosyl umbelliferone-PB (GUPB), is nonfluorescent under conditions of the assay; however,
Substrate-labeled fluorescent immunoassay for measuring dibekacin concentrations in serum and plasma.
Journal:Antimicrob Agents Chemother
PubMed ID:6357071
'We have developed a homogeneous substrate-labeled fluorescent immunoassay for the measurement of dibekacin concentrations in serum and plasma. The fluorogenic enzyme substrate beta-galactosyl-umbelliferone was covalently attached to tobramycin, an aminoglycoside structurally similar to dibekacin, to prepare a fluorogenic drug reagent (FDR). The FDR is nonfluorescent under assay conditions, but fluoresces
A homogeneous fluorescent immunoassay for human immunoglobulin M.
Journal:Clin Chem
PubMed ID:7014030
'We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensity of the labeled IgM at 450 nm (with excitation at 400 nm) is negligible, but when
Substrate-labeled fluorescent immunoassay for phenytoin in human serum.
Journal:Clin Chem
PubMed ID:108033
'A homogeneous substrate-labeled fluorescent immunoassay has been applied to the measurement of phenytoin concentrations in human serum. We coupled a fluorogenic enzyme substrate, galactosyl-umbelliferone, covalently to a derivative of phenytoin. Under assay conditions, this drug-substrate conjugate was nonfluorescent but became fluorescent upon hydrolysis catalyzed by bacterial beta-galactosidase. When antibody to
Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.
Journal:J Virol
PubMed ID:12388712
'We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from