Substrate-labeled fluorescent immunoassay for phenytoin in human serum.
Authors:Wong RC, Burd JF, Carrico RJ, Buckler RT, Thoma J, Boguslaski RC
Journal:Clin Chem
PubMed ID:108033
'A homogeneous substrate-labeled fluorescent immunoassay has been applied to the measurement of phenytoin concentrations in human serum. We coupled a fluorogenic enzyme substrate, galactosyl-umbelliferone, covalently to a derivative of phenytoin. Under assay conditions, this drug-substrate conjugate was nonfluorescent but became fluorescent upon hydrolysis catalyzed by bacterial beta-galactosidase. When antibody to ... More