FluoReporter™ lacZ/半乳糖苷酶定量试剂盒
FluoReporter&trade; <i>lacZ</i>/半乳糖苷酶定量试剂盒
Invitrogen™

FluoReporter™ lacZ/半乳糖苷酶定量试剂盒

FluoReporter™ lacZ/半乳糖苷酶定量试剂盒包括 β-半乳糖苷酶底物、3-羧基香豆素 β-D-半乳吡喃糖苷,可产生蓝色荧光水解产物 7-羟基香豆素-3-羧酸了解更多信息
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货号数量
F29051 Kit
货号 F2905
价格(CNY)
5,108.00
Each
添加至购物车
数量:
1 Kit
价格(CNY)
5,108.00
Each
添加至购物车
FluoReporter™ lacZ/半乳糖苷酶定量试剂盒包括 β-半乳糖苷酶底物、3-羧基香豆素 β-D-半乳吡喃糖苷,可产生蓝色荧光水解产物 7-羟基香豆素-3-羧酸。
仅供科研使用。不可用于诊断程序。
规格
检测β-Gal (lacZ) 检测
检测方法荧光
产品线FluoReporter
产品类型β-半乳糖苷酶报告基因测定系统
数量1 Kit
运输条件室温
底物CUG
底物属性化学底物
底物类型β-Gal 底物
目标酶β-半乳糖苷酶
产品规格试剂盒
Unit SizeEach
内容与储存
在冷冻冰箱(-5 至 -30°C)中避光储存。

引用和文献 (22)

引用和文献
Abstract
Substrate-labeled fluorescent immunoassay for phenobarbital.
Authors:Krausz LM, Hitz JB, Buckler RT, Burd JF
Journal:Ther Drug Monit
PubMed ID:7013165
An assay for the anticonvulsant drug phenobarbital (PB) has been developed that is based on the principles of the substrate-labeled fluorescent immunoassay. A fluorogenic enzyme substrate, galactosyl umbelliferone, was covalently linked to a derivative of PB. The labeled drug, galactosyl umbelliferone-PB (GUPB), is nonfluorescent under conditions of the assay; however, ... More
Substrate-labeled fluorescent immunoassay for measuring dibekacin concentrations in serum and plasma.
Authors:Place JD, Thompson SG
Journal:Antimicrob Agents Chemother
PubMed ID:6357071
'We have developed a homogeneous substrate-labeled fluorescent immunoassay for the measurement of dibekacin concentrations in serum and plasma. The fluorogenic enzyme substrate beta-galactosyl-umbelliferone was covalently attached to tobramycin, an aminoglycoside structurally similar to dibekacin, to prepare a fluorogenic drug reagent (FDR). The FDR is nonfluorescent under assay conditions, but fluoresces ... More
A homogeneous fluorescent immunoassay for human immunoglobulin M.
Authors:Worah D, Yeung KK, Ward FE, Carrico RJ
Journal:Clin Chem
PubMed ID:7014030
'We describe a homogeneous substrate-labeled fluorescent immunoassay for human IgM. In this competitive-binding method we use a fluorogenic substrate for Escherichia coli beta-galactosidase, N-(6-aminohexyl)-7-beta-galactosyl-coumarin-3-carboxamide, which is covalently coupled to IgM. The fluorescence emission intensity of the labeled IgM at 450 nm (with excitation at 400 nm) is negligible, but when ... More
Substrate-labeled fluorescent immunoassay for phenytoin in human serum.
Authors:Wong RC, Burd JF, Carrico RJ, Buckler RT, Thoma J, Boguslaski RC
Journal:Clin Chem
PubMed ID:108033
'A homogeneous substrate-labeled fluorescent immunoassay has been applied to the measurement of phenytoin concentrations in human serum. We coupled a fluorogenic enzyme substrate, galactosyl-umbelliferone, covalently to a derivative of phenytoin. Under assay conditions, this drug-substrate conjugate was nonfluorescent but became fluorescent upon hydrolysis catalyzed by bacterial beta-galactosidase. When antibody to ... More
Adenovirus-facilitated nuclear translocation of adeno-associated virus type 2.
Authors:Xiao W, Warrington KH, Hearing P, Hughes J, Muzyczka N
Journal:J Virol
PubMed ID:12388712
'We examined cytoplasmic trafficking and nuclear translocation of adeno-associated virus type 2 (AAV) by using Alexa Fluor 488-conjugated wild-type AAV, A20 monoclonal antibody immunocytochemistry, and subcellular fractionation techniques followed by DNA hybridization. Our results indicated that in the absence of adenovirus (Ad), AAV enters the cell rapidly and escapes from ... More