Designed auto-assembly of nanostreptabodies for rapid tissue-specific targeting in vivo.
AuthorsValadon P, Darsow B, Buss TN, Czarny M, Griffin NM, Nguyen HN, Oh P, Borgstrom P, Chrastina A, Schnitzer JE,
JournalJ Biol Chem
PubMed ID19850928
Molecular medicine can benefit greatly from antibodies that deliver therapeutic and imaging agents to select organs and diseased tissues. Yet the development of complex and defined composite nanostructures remains a challenge that requires both designed stoichiometric assembly and superior in vivo testing ability. Here, we generate nanostructures called nanostreptabodies by ... More
Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization.
AuthorsAndrews NL, Lidke KA, Pfeiffer JR, Burns AR, Wilson BS, Oliver JM, Lidke DS,
JournalNat Cell Biol
PubMed ID18641640
The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane components. Here, simultaneous observations of quantum dot-labelled FcepsilonRI motion and GFP-tagged actin dynamics provide direct evidence that actin filament bundles define micron-sized domains that confine mobile receptors. Dynamic reorganization of actin structures occurs over seconds, making the location ... More
Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids.
AuthorsBatchelor RH, Sarkez A, Cox WG, Johnson I
JournalBiotechniques
PubMed ID18019342
As a component of the (strept)avidin affinity system, biotin is often covalently linked to proteins or nucleic acids. We describe here a microplate-based high-throughput fluorometric assay for biotin linked to either proteins or nucleic acids based on fluorescence resonance energy transfer (FRET). This assay utilizes a complex of Alexa Fluoro ... More
One at a time, live tracking of NGF axonal transport using quantum dots.
AuthorsCui B, Wu C, Chen L, Ramirez A, Bearer EL, Li WP, Mobley WC, Chu S
JournalProc Natl Acad Sci U S A
PubMed ID17698956
Retrograde axonal transport of nerve growth factor (NGF) signals is critical for the survival, differentiation, and maintenance of peripheral sympathetic and sensory neurons and basal forebrain cholinergic neurons. However, the mechanisms by which the NGF signal is propagated from the axon terminal to the cell body are yet to be ... More
Coupling of biotin-(poly(ethylene glycol))amine to poly(D,L-lactide-co-glycolide) nanoparticles for versatile surface modification.
AuthorsWeiss B, Schneider M, Muys L, Taetz S, Neumann D, Schaefer UF, Lehr CM
JournalBioconjug Chem
PubMed ID17590034
Generally, polymeric nanoparticles (NP) for drug targeting are designed to entrap the drug moiety in the core and to present the targeting moiety on the surface. However, in most cases, common preparation techniques of polymeric NP need to be specifically arranged for each compound to be entrapped or attached. In ... More