Targeted cellular delivery of quantum dots loaded on and in biotinylated liposomes.
AuthorsSigot V, Arndt-Jovin DJ, Jovin TM,
JournalBioconjug Chem
PubMed ID20715851
'We describe the preparation, biophysical characterization, and receptor-mediated cellular internalization of biotinylated lipid particles (BLPs) loaded on the surface and internally with two distinct (colors) of quantum dot (QD) probes. BLPs were formulated with 1.4 and 2.7 mol % PEG-lipids containing either a fusogenic or pH-sensitive lipid to promote bilayer ... More
Chromosomal biomarkers for detection of human papillomavirus associated genomic instability in epithelial cells of cervical cytology specimens.
AuthorsSokolova I, Algeciras-Schimnich A, Song M, Sitailo S, Policht F, Kipp BR, Voss JS, Halling KC, Ruth A, King W, Underwood D, Brainard J, Morrison L,
JournalJ Mol Diagn
PubMed ID17975027
The goal of this study was to compare how accumulation of chromosomal aberrations in human papillomavirus (HPV)-infected cells correlates with the severity of cervical dysplastic lesions. We assessed the frequency of genomic alterations for 35 different loci in a pilot biopsy study and selected two loci (3q26 and 8q24) with ... More
Fluorometric assay for quantitation of biotin covalently attached to proteins and nucleic acids.
AuthorsBatchelor RH, Sarkez A, Cox WG, Johnson I
JournalBiotechniques
PubMed ID18019342
As a component of the (strept)avidin affinity system, biotin is often covalently linked to proteins or nucleic acids. We describe here a microplate-based high-throughput fluorometric assay for biotin linked to either proteins or nucleic acids based on fluorescence resonance energy transfer (FRET). This assay utilizes a complex of Alexa Fluoro ... More