FISH Tag™ DNA Kits with Alexa Fluor™ Dyes
FISH Tag™ DNA Kits with Alexa Fluor™ Dyes
Invitrogen™

FISH Tag™ DNA Kits with Alexa Fluor™ Dyes

FISH Tag DNA kits provide a complete solution for fluorescence in situ hybridization (FISH) applications. Choose from kits using Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, or multicolor dyes for probe synthesis, purification, and labeling of specimens for imaging.
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货号标签或染料颜色
F32948Alexa Fluor™ 555橙色
F32947Alexa Fluor™ 488绿色
F32949Alexa Fluor™ 594红色
F32951Alexa Fluor™ 488、Alexa Fluor™ 555、Alexa Fluor™ 594、Alexa Fluor™ 647, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647Multi-color: Green, Orange, Red, Far-Red
货号 F32948
价格(CNY)
9,061.00
Each
添加至购物车
标签或染料:
Alexa Fluor™ 555
颜色:
橙色
价格(CNY)
9,061.00
Each
添加至购物车

Choose from three, single-color FISH Tag DNA kits with Alexa Fluor dye options (green, orange, or red). The FISH DNA multicolor kit contains four spectrally distinct dyes (green, orange, red, and deep-red) to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and in situ analysis of mitochondrial DNA localization and content. All kits include 16 reagents and come with a comprehensive, detailed protocol to help ensure your success.

FISH Tag™ DNA Kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. Choose from FISH Tag™ DNA Kits in three Alexa Fluor™ colors or get the FISH Tag™ DNA Multicolor Kit (Cat. No. F32951), which contains four spectrally distinct dyes to allow simultaneous localization of multiple sequence-specific DNA targets in chromosomal spreads and for in situ analysis of mitochondrial DNA localization and content. All kits include 16 reagents and come with a comprehensive, detailed protocol to help ensure your success.

FISH Tag™ Kit Specifications:

  • Dye (Ex/Em): Alexa Fluor™ 594 (590/615 nm)
  • Comprehensive kit includes 16 reagents
  • Comes with detailed protocol to help ensure your success

Complete Workflow Solution for FISH

FISH Tag™ Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor™ dyes. PureLink™ nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade™ Gold antifade reagent is added to preserve the fluorescent signal during imaging.

More Options for Nucleic Acid Labeling

To review various options for nucleic acid labeling (including DNA and RNA FISH), review Labeling Oligonucleotides and Nucleic Acids—Section 8.2 in the Molecular Probes™ Handbook.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.
规格
颜色橙色
激发/发射555/565 nm
适用于(应用)FISH Tag, fluorescent microscopy, DNA In situ hybridization
反应次数10 Reactions
产品线Alexa Fluor™、FISH Tag™
产品类型DNA 原位杂交测定试剂盒
数量1 Kit
运输条件干冰
靶标DNA
靶标特异性无靶标特异性
检测方法荧光
最终产品类型Probes (Labeled DNA), cDNA (Labeled)
产品规格试剂盒
标记目标DNA (General), cDNA
标签或染料Alexa Fluor™ 555
样品类型DNA, Tissue (FFPE), Tissue (CRYO), Cell Culture Cells
Unit SizeEach
内容与储存
在冰箱(-5 至 -30°C)中避光储存。

常见问题解答 (FAQ)

For imaging, what are the limitations of FISH probes?

FISH probes are generally from 300 to 1500 bp in length. To adequately image a gene or other target, the FISH probes must cover a contiguous length of ˜10 kbp. Shorter strand lengths or low-abundance DNA or RNA targets may be detected with the use of fluorescent amplification methods, such as tyramide signal amplification (TSA). For more information using TSA to amplify FISH detection, see this manual (http://tools.thermofisher.com/content/sfs/manuals/mp20912.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

When should I use an RNA FISH probe versus a DNA FISH probe?

RNA FISH probes are the best option for hybridization to mRNA, rRNA, or other RNA targets. DNA FISH probes can also be used to detect RNA targets, but they do not hybridize as well to mRNA and other RNA target molecules. DNA FISH probes are routinely used to detect gene loci on chromosomes. RNA FISH probes can also be used to detect genes on chromosomes, but DNA FISH probes are more robust for this application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.