I used one of your FluoroMyelin stains and noticed it stains cells other than glial cells. Is there something wrong with the product?
FluoroMyelin is a lipid stain, any lipid can be stained by it but there is a higher lipid content in myelin that it will stain much more intensely than other membranes.
'The misfolding of the prion protein (PrP(c)) is a central event in prion diseases, yet the normal function of PrP(c) remains unknown. PrP(c) has putative roles in many cellular processes including signaling, survival, adhesion, and differentiation. Given the abundance of PrP(c) in the developing and mature mammalian CNS, we investigated ... More
Rescuing qkV dysmyelination by a single isoform of the selective RNA-binding protein QKI.
Authors:Zhao L, Tian D, Xia M, Macklin WB, Feng Y,
Journal:J Neurosci
PubMed ID:17079655
Alternative splicing of the qkI transcript generates multiple isoforms of the selective RNA-binding protein QKI, which play key roles in controlling the homeostasis of their mRNA targets. QKI deficiency in oligodendrocytes of homozygous quakingviable (qkV/qkV) mutant mice results in severe hypomyelination, indicating the essential function of QKI in myelinogenesis. However, ... More
Neuronal glutathione deficiency and age-dependent neurodegeneration in the EAAC1 deficient mouse.
Authors:Aoyama K, Suh SW, Hamby AM, Liu J, Chan WY, Chen Y, Swanson RA
Journal:Nat Neurosci
PubMed ID:16311588
Uptake of the neurotransmitter glutamate is effected primarily by transporters expressed on astrocytes, and downregulation of these transporters leads to seizures and neuronal death. Neurons also express a glutamate transporter, termed excitatory amino acid carrier-1 (EAAC1), but the physiological function of this transporter remains uncertain. Here we report that genetically ... More
Collagen XXVIII, a novel von Willebrand factor A domain-containing protein with many imperfections in the collagenous domain.
Authors:Veit G, Kobbe B, Keene DR, Paulsson M, Koch M, Wagener R
Journal:J Biol Chem
PubMed ID:16330543
Here we describe a novel collagen belonging to the class of von Willebrand factor A (VWA) domain-containing proteins. This novel protein was identified by screening the EST data base and was subsequently recombinantly expressed and characterized as an authentic tissue component. The COL28A1 gene on human chromosome 7p21.3 and on ... More
Skin and gut imprinted helper T cell subsets exhibit distinct functional phenotypes in central nervous system autoimmunity.