<i>Funga</i>Light™ 酵母菌 CFDA、AM/碘化丙啶活力试剂盒

Invitrogen™

FungaLight™ 酵母菌 CFDA、AM/碘化丙啶活力试剂盒

FungaLight™ 酵母菌 CFDA、AM/碘化丙啶活力试剂盒

货号	数量
F34953	200 kit

货号 F34953

价格（CNY)

6,878.00

Ends: 31-Dec-2026

9,139.00

共减 2,261.00 (25%)

200 kit

价格（CNY)

6,878.00

Ends: 31-Dec-2026

9,139.00

共减 2,261.00 (25%)

The FungaLight™ Yeast CFDA, AM / Propidium Iodide Vitality Kit combines a cell permeable esterase substrate with a membrane integrity indicator to evaluate the vitality of yeast cells by flow cytometry or microscopy. The acetoxymethyl ester (AM) of the esterase substrate 5-carboxyfluorescein diacetate (CFDA) allows this reagent to permeant cell membranes Once inside the cell, the lipophilic blocking and diacetate groups are cleaved by nonspecific esterases, resulting in a fluorescent, charged form that leaks out of cells very slowly. In contrast, the membrane integrity indicator, propidium iodide penetrates yeast with damaged membranes. With an appropriate mixture of the CFDA, AM and propidium iodide stains, esterase-active yeast with intact cell membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

FungaLight™ 酵母菌 CFDA、AM/碘化丙啶活力试剂盒结合了细胞渗透性酯酶底物和细胞膜完整性指示剂，从而利用流式细胞分析或显微镜检查对酵母细胞活力进行评价。酯酶底物 5-羧基荧光素二乙酸酯 (CFDA) 的乙酰氧基甲基酯 (AM) 可使该试剂透过细胞膜。进入细胞后，亲脂性保护基团和二乙酸酯基团被非特异性酯酶切割，从而产生带电荷形式的荧光（非常缓慢地从细胞中泄漏）。相反，膜完整性指示剂（碘化丙啶）能够穿透具有受损细胞膜的酵母细胞。通过使用 CFDA AM 和碘化丙啶染料的混合试剂染色，具有完整细胞膜的酯酶活性酵母细胞会被染上绿色荧光，而具有受损细胞膜的酵母细胞则会被染上红色荧光。

仅供科研使用。不可用于诊断程序。

细胞类型酵母细胞

检测方法荧光

染料类型其他标记或染料

产品规格管、玻片

数量200 kit

运输条件室温

溶解度DMSO（二甲亚砜）

发射波长范围PI：490⁄635，CFDA：492⁄517

适用于（应用）活力测定试剂盒

适用于（设备）荧光显微镜, 流式细胞仪

产品线FungaLight

产品类型AM/碘化丙啶细胞活力试剂盒

Unit SizeEach

内容与储存

包含 3 瓶 5-CFDA（1 mg/瓶）、1 瓶碘化丙啶和 1 瓶 DMSO。在冰箱（-5 至 -30°C）中避光储存。

常见问题解答 (FAQ)

我可通过流式细胞仪观察细菌和酵母么？

可以。流式细胞仪可用于：

•计数检测：用于流式细胞仪的细菌计数试剂盒（货号B7277）或LIVE/DEAD BacLight细菌活性和计数试剂盒（货号L34856）
•活性/活力检测：LIVE/DEAD BacLight 细菌活性试剂盒（货号 L13152）
•膜电位：BacLight细菌膜电位试剂盒（货号B34950）
•酵母活性/活力实验：用于流式细胞仪（货号L34952）的LIVE/DEAD FungaLight酵母活性试剂盒，FungaLight酵母CFDA, AM/碘化丙啶活力试剂盒（货号F34953）。

Can I look at bacteria and yeast on a flow cytometer?

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

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