A developmental switch in neurotransmitter flux enhances synaptic efficacy by affecting AMPA receptor activation.
AuthorsRenger JJ, Egles C, Liu G
JournalNeuron
PubMed ID11239436
'Formation of glutamatergic synapses entails development of "silent" immature contacts into mature functional synapses. To determine how this transformation occurs, we investigated the development of neurotransmission at single synapses in vitro. Maturation of presynaptic function, assayed with endocytotic markers, followed accumulation of synapsin I. During this period, synaptic transmission was ... More
Directed cell growth on protein-functionalized hydrogel surfaces.
AuthorsHynd MR, Frampton JP, Dowell-Mesfin N, Turner JN, Shain W
JournalJ Neurosci Methods
PubMed ID17368788
'Biochemical surface modification has been used to direct cell attachment and growth on a biocompatible gel surface. Acrylamide-based hydrogels were photo-polymerized in the presence of an acroyl-streptavidin monomer to create planar, functionalized surfaces capable of binding biotin-labelled proteins. Soft protein lithography (microcontact printing) of proteins was used to transfer the ... More
CD24 is expressed by myofiber synaptic nuclei and regulates synaptic transmission.
AuthorsJevsek M, Jaworski A, Polo-Parada L, Kim N, Fan J, Landmesser LT, Burden SJ
JournalProc Natl Acad Sci U S A
PubMed ID16606832
'The genes encoding several synaptic proteins, including acetylcholine receptors, acetylcholinesterase, and the muscle-specific kinase, MuSK, are expressed selectively by a small number of myofiber nuclei positioned near the synaptic site. Genetic analysis of mutant mice suggests that additional genes, expressed selectively by synaptic nuclei, might encode muscle-derived retrograde signals that ... More
Constitutive sharing of recycling synaptic vesicles between presynaptic boutons.
AuthorsDarcy KJ, Staras K, Collinson LM, Goda Y
JournalNat Neurosci
PubMed ID16462738
'The synaptic vesicle cycle is vital for sustained neurotransmitter release. It has been assumed that functional synaptic vesicles are replenished autonomously at individual presynaptic terminals. Here we tested this assumption by using FM dyes in combination with fluorescence recovery after photobleaching and correlative light and electron microscopy in cultured rat ... More
Using FM1-43 to study neuropeptide granule dynamics and exocytosis.
In the study of neuropeptide secretion and membrane trafficking, the fluorescent dye FM1-43 provides the ability to label selectively those structures that are undergoing exocytosis and endocytosis in living cells in real time. This review describes the unique properties of the FM dyes that make them ideal for studying neuropeptide ... More
FM 1-43 labeling of synaptic vesicle pools at the Drosophila neuromuscular junction.
AuthorsVerstreken P, Ohyama T, Bellen HJ,
JournalMethods Mol Biol
PubMed ID18369958
To maintain transmitter release during intense stimulation, neurons need to efficiently recycle vesicles at the synapse. Following membrane fusion, vesicles are reshaped and formed from the plasma membrane by bulk or clathrin-mediated endocytosis. Most synapses, including the Drosophila neuromuscular junction (NMJ), can also recycle synaptic vesicles directly by closing the ... More
The color of lactotroph secretory granules stained with FM1-43 depends on dye concentration.
AuthorsJohnson JM, Betz WJ,
JournalBiophys J
PubMed ID18065476
When pituitary lactotroph granules undergo exocytosis in the presence of FM1-43, their cores absorb dye and fluoresce brightly. We report that different granules fluoresce with different colors, despite being stained with a single fluorescent dye; emission spectra from individual granules show up to a 25 nm difference between the greenest ... More
Angiogenesis: an improved in vitro biological system and automated image-based workflow to aid identification and characterization of angiogenesis and angiogenic modulators.
AuthorsSantos AF, Zaltsman AB, Martin RC, Kuzmin A, Alexandrov Y, Roquemore EP, Jessop RA, van Erck MG, Verheijen JH,
JournalAssay Drug Dev Technol
PubMed ID19035850
Angiogenesis is a general term describing formation of new tube-like microvessel sprouts that are the size of capillary blood vessels. Angiogenesis is fundamental in key stages of embryonic development, organ formation, and wound repair and is also involved in the development and progression of a variety of pathological conditions, including ... More
Endocytic trafficking from the small intestinal brush border probed with FM dye.
The small intestinal brush border functions as the body's main portal for uptake of dietary nutrients and simultaneously acts as the largest permeability barrier against pathogens. To enable this, the digestive enzymes of the brush border are organized in lipid raft microdomains stabilized by cross-linking galectins and intelectin, but little ... More
Heterocellular contact at the myoendothelial junction influences gap junction organization.
AuthorsIsakson BE, Duling BR
JournalCirc Res
PubMed ID15961721
Heterocellular communication between vascular smooth muscle cells (VSMC) and endothelial cells (EC) at the myoendothelial junction (MEJ) is a critical part of control of the arteriolar wall. We have developed an in vitro model of the MEJ composed of primary cultures of murine EC and VSMC. Immunoctytochemistry and immunoblots demonstrated ... More
Streamlined synaptic vesicle cycle in cone photoreceptor terminals.
AuthorsRea R, Li J, Dharia A, Levitan ES, Sterling P, Kramer RH
JournalNeuron
PubMed ID15003175
Cone photoreceptors tonically release neurotransmitter in the dark through a continuous cycle of exocytosis and endocytosis. Here, using the synaptic vesicle marker FM1-43, we elucidate specialized features of the vesicle cycle. Unlike retinal bipolar cell terminals, where stimulation triggers bulk membrane retrieval, cone terminals appear to exclusively endocytose small vesicles. ... More
Membrane retrieval in neutrophils during phagocytosis: inhibition by M protein-expressing S. pyogenes bacteria.
AuthorsBauer S, Tapper H
JournalJ Leukoc Biol
PubMed ID15339937
During phagocytosis and phagosome maturation, complex membrane traffic events must be coordinated. We have observed, using fluorescent fluid-phase and membrane markers, that in the human neutrophil, internalization of nonopsonized, Gram-positive bacteria, but not of latex beads, is accompanied by a rapid and localized formation of pinosomal structures. This pinocytic response ... More
Methods for detecting internalized, FM 1-43 stained particles in epithelial cells and monolayers.
AuthorsBertrand CA, Laboisse C, Hopfer U, Bridges RJ, Frizzell RA
JournalBiophys J
PubMed ID16935947
The membrane dye FM 1-43 has frequently been used to quantify exocytosis in neurons. In epithelia, intense lateral intracellular space staining and fluctuations in baseline labeling produced inconsistent results. Membrane retrieved in the presence of FM 1-43 retains the dye, however, and cells that undergo compensatory endocytosis during and following ... More
Glutathione export during apoptosis requires functional multidrug resistance-associated proteins.
AuthorsHammond CL, Marchan R, Krance SM, Ballatori N
JournalJ Biol Chem
PubMed ID17374608
GSH is released in cells undergoing apoptosis, and the present study indicates that the multidrug resistance-associated proteins (MRPs/ABCC) are responsible for this GSH release. Jurkat cells released approximately 75-80% of their total intracellular GSH during both Fas antibody- and staurosporine-induced apoptosis. In contrast, Raji cells, a lymphocyte cell line that ... More
Distinct progenitor populations mediate regeneration in the zebrafish lateral line.
AuthorsThomas ED, Raible DW
JournalElife
PubMed ID30834891
'Mechanosensory hair cells of the zebrafish lateral line regenerate rapidly following damage. These renewed hair cells arise from the proliferation of surrounding support cells, which undergo symmetric division to produce two hair cell daughters. Given the continued regenerative capacity of the lateral line, support cells presumably have the ability to ... More
Role of TRPM8 Channels in Altered Cold Sensitivity of Corneal Primary Sensory Neurons Induced by Axonal Damage.
AuthorsPiña R, Ugarte G, Campos M, Íñigo-Portugués A, Olivares E, Orio P, Belmonte C, Bacigalupo J, Madrid R
JournalJ Neurosci
PubMed ID31471469
'The cornea is extensively innervated by trigeminal ganglion cold thermoreceptor neurons expressing TRPM8 (transient receptor potential cation channel subfamily M member 8). These neurons respond to cooling, hyperosmolarity and wetness of the corneal surface. Surgical injury of corneal nerve fibers alters tear production and often causes dry eye sensation. The ... More
MicroRNA-153 impairs presynaptic plasticity by blocking vesicle release following chronic brain hypoperfusion.
AuthorsYan ML, Zhang S, Zhao HM, Xia SN, Jin Z, Xu Y, Yang L, Qu Y, Huang SY, Duan MJ, Mao M, An XB, Mishra C, Zhang XY, Sun LH, Ai J
JournalCell Commun Signal
PubMed ID32252776
'Chronic brain hypoperfusion (CBH) is closely related to Alzheimer''s disease (AD) and vascular dementia (VaD). Meanwhile, synaptic pathology plays a prominent role in the initial stage of AD and VaD. However, whether and how CBH impairs presynaptic plasticity is currently unclear.' ... More
The CHD Protein, Kismet, is Important for the Recycling of Synaptic Vesicles during Endocytosis.
AuthorsLatcheva NK, Delaney TL, Viveiros JM, Smith RA, Bernard KM, Harsin B, Marenda DR, Liebl FLW
JournalSci Rep
PubMed ID31852969
'Chromatin remodeling proteins of the chromodomain DNA-binding protein family, CHD7 and CHD8, mediate early neurodevelopmental events including neural migration and differentiation. As such, mutations in either protein can lead to neurodevelopmental disorders. How chromatin remodeling proteins influence the activity of mature synapses, however, is relatively unexplored. A critical feature of ... More
Bile acids and ceramide overcome the entry restriction for GII.3 human norovirus replication in human intestinal enteroids.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID31896578
High expression levels of the D686N Parkinson's disease mutation in VPS35 induces a-synuclein-dependent toxicity in yeast.
AuthorsHuang Y, Chen X, He X, Guo C, Sun X, Liang F, Long S, Lu X, Feng L, Guo W, Zeng Y, Pei Z
JournalMol Med Rep
PubMed ID28487947
'Parkinson''s disease (PD) is a common neurodegenerative disorder that affects ~2% of the human population aged >65. a-synuclein serves a role in the pathogenesis of PD as it is a primary component of Lewy bodies, a pathological feature of PD. Endosomal-lysosomal dysfunction may be a key factor involved in the ... More
Transcriptional Dynamics of Hair-Bundle Morphogenesis Revealed with CellTrails.
Authors
JournalCell Rep
PubMed ID29874578
The Power of Human Protective Modifiers: PLS3 and CORO1C Unravel Impaired Endocytosis in Spinal Muscular Atrophy and Rescue SMA Phenotype.
Authors
JournalAm J Hum Genet
PubMed ID27499521
SLC-30A9 is required for Zn2+ homeostasis, Zn2+ mobilization, and mitochondrial health.
Authors
JournalProc Natl Acad Sci U S A
PubMed ID34433664
Surface-associated antigen induces permeabilization of primary mouse B-cells and lysosome exocytosis facilitating antigen uptake and presentation to T-cells.
Authors
JournalElife
PubMed ID34704555
Primary cilia regulate mTORC1 activity and cell size through Lkb1.
Adaptive cell invasion maintains lateral line organ homeostasis in response to environmental changes.
Authors
JournalDev Cell
PubMed ID33878346
A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission.
Authors
JournalNeuron
PubMed ID30704911
Pericyte-to-endothelial cell signaling via vitronectin-integrin regulates blood-CNS barrier.
Authors
JournalNeuron
PubMed ID35294899
Immunostaining in whole-mount lipid-cleared peripheral nerves and dorsal root ganglia after neuropathy in mice.
AuthorsBernal L, Cisneros E, García-Magro N, Roza C
JournalSci Rep
PubMed ID31182787
Immunohistochemical characterization of primary afferent fibers (intact or after nerve damage) is traditionally performed in thin sections from dorsal root ganglia (DRGs) or in teased fibers, as light scattering in whole-mounts compromises visualization. These procedures are time-consuming, require specific equipment and advanced experimental skills. Lipid-clearing techniques are increasing in popularity, ... More
Phenotypic Drug Screening for Dysferlinopathy Using Patient-Derived Induced Pluripotent Stem Cells.
AuthorsKokubu Y, Nagino T, Sasa K, Oikawa T, Miyake K, Kume A, Fukuda M, Fuse H, Tozawa R, Sakurai H
JournalStem Cells Transl Med
PubMed ID31250983
Dysferlinopathy is a progressive muscle disorder that includes limb-girdle muscular dystrophy type 2B and Miyoshi myopathy (MM). It is caused by mutations in the dysferlin (DYSF) gene, whose function is to reseal the muscular membrane. Treatment with proteasome inhibitor MG-132 has been shown to increase misfolded dysferlin in fibroblasts, allowing ... More
Retromer subunit, VPS29, regulates synaptic transmission and is required for endolysosomal function in the aging brain.
AuthorsYe H, Ojelade SA, Li-Kroeger D, Zuo Z, Wang L, Li Y, Gu JY, Tepass U, Rodal AA, Bellen HJ, Shulman JM
JournalElife
PubMed ID32286230
Retromer, including Vps35, Vps26, and Vps29, is a protein complex responsible for recycling proteins within the endolysosomal pathway. Although implicated in both Parkinson's and Alzheimer's disease, our understanding of retromer function in the adult brain remains limited, in part because ... More
Rapid 3D Enhanced Resolution Microscopy Reveals Diversity in Dendritic Spinule Dynamics, Regulation, and Function.
AuthorsZaccard CR, Shapiro L, Martin-de-Saavedra MD, Pratt C, Myczek K, Song A, Forrest MP, Penzes P
JournalNeuron
PubMed ID32464088
Dendritic spinules are thin protrusions, formed by neuronal spines, not adequately resolved by diffraction-limited light microscopy, which has limited our understanding of their behavior. Here we performed rapid structured illumination microscopy and enhanced resolution confocal microscopy to study spatiotemporal spinule dynamics in cortical pyramidal neurons. Spinules recurred at the same ... More
Francisella novicida and F. philomiragia biofilm features conditionning fitness in spring water and in presence of antibiotics.
AuthorsSiebert C, Villers C, Pavlou G, Touquet B, Yakandawala N, Tardieux I, Renesto P
JournalPLoS One
PubMed ID32023304
Biofilms are currently considered as a predominant lifestyle of many bacteria in nature. While they promote survival of microbes, biofilms also potentially increase the threats to animal and public health in case of pathogenic species. They not only facilitate bacteria transmission and persistence, but also promote spreading of antibiotic resistance ... More
Lack of PDZD7 long isoform disrupts ankle-link complex and causes hearing loss in mice.
AuthorsDu H, Zou L, Ren R, Li N, Li J, Wang Y, Sun J, Yang J, Xiong W, Xu Z
JournalFASEB J
PubMed ID31914662
Usher syndrome (USH) is the most frequent form of combined hereditary deafness-blindness, characterized by hearing loss and retinitis pigmentosa, with or without vestibular dysfunction. PDZD7 is a PDZ domain-containing scaffold protein that was suggested to be a USH modifier and a contributor to digenic USH. In the inner ear hair ... More