Novel fluo-4 analogs for fluorescent calcium measurements.
AuthorsMartin VV, Beierlein M, Morgan JL, Rothe A, Gee KR
JournalCell Calcium
PubMed ID15488600
We report new fluorescent calcium indicators based on fluo-4. Attachment of a carboxamide or methylenecarboxamide moiety to the BAPTA chelator portion of fluo-4 allowed for the attachment of dextrans, protein-reactive moieties, and biotin. In particular, a high affinity fluo-4 dextran conjugate was prepared and shown to be functional in brain ... More