Fluo-4 NW 钙含量检测试剂盒
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Fluo-4 NW 钙含量检测试剂盒
引用和文献 (21)
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Fluo-4 NW 钙含量检测试剂盒

Fluo-4 NW（免洗涤）钙含量测定试剂盒提供了专有的钙含量测定配方，既不需要洗涤步骤，也不需要淬灭基团染料。与包含洗涤步骤的标准 fluo-3 和 fluo-4 测定试剂盒相比，fluo-4 NW了解更多信息
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货号数量
F3620610 个微孔板
货号 F36206
价格（CNY)
3,639.00
飞享价
Ends: 31-Dec-2025
4,899.00
共减 1,260.00 (26%)
Each
添加至购物车
数量:
10 个微孔板
价格（CNY)
3,639.00
飞享价
Ends: 31-Dec-2025
4,899.00
共减 1,260.00 (26%)
Each
添加至购物车
Fluo-4 NW（免洗涤）钙含量测定试剂盒提供了专有的钙含量测定配方，既不需要洗涤步骤，也不需要淬灭基团染料。与包含洗涤步骤的标准 fluo-3 和 fluo-4 测定试剂盒相比，fluo-4 NW 测定试剂盒能够达到更高的荧光强度。与标准 fluo-4 测定试剂盒相比，取消洗涤步骤降低了变异性并提高了 Z´ 值，同时实现了更简单、更快速的测定。

fluo-4NW 指示剂在 pH 值为 7–7.5 的缓冲液中不发出荧光且能够保持稳定数小时，因此自发转化为对 Ca2+ 敏感的形式不是产生背景荧光的主要原因。将指示剂染料加入反应孔前除去培养基可消除生长培养基（例如，酯酶活性、与感兴趣受体相互作用的蛋白质、或酚红）对基线荧光的贡献。

细胞外潜在荧光形成的另一个原因是指示剂被有机阴离子转运蛋白排出细胞外。通常使用丙磺舒抑制这种转运并降低基线信号。我们合成了专有的水溶性丙磺舒，随 Fluo-4 NW 钙含量测定试剂盒配套提供。这种形式的丙磺舒的优点是易于溶解在缓冲液中，并且其使用比游离酸更安全，因为游离酸需要具有腐蚀性的 1 M 氢氧化钠才能溶解。Fluo-4 NW 钙含量测定试剂盒设计用于微孔板和 HTS，且可对贴壁细胞和不贴壁细胞进行测定。

Fluo-4 AM 是一种荧光 Ca2+ 指示剂，广泛用于高通量筛选 (HTS) 应用以对激动剂刺激的和拮抗剂抑制的钙信号转导进行细胞内测量。凭借可见波长激发（兼容氩离子激光源）、高灵敏度以及结合 Ca2+ 后荧光显著增强的优势，其成为表征 G 蛋白偶联受体 (GPCR) 药理学和功能的首选指示剂。因为具有这些特性，fluo-4 AM 不仅在微孔板筛选应用方面具有吸引力，在显微镜检查和流式细胞分析方面也同样具有吸引力。

仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
染料类型基于荧光染料
数量10 个微孔板
运输条件室温
适用于（应用）钙含量测定试剂盒
适用于（设备）微孔板读数仪
产品类型染色剂
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中避光储存。

引用和文献 (21)

引用和文献
Abstract
Cell death and autophagy under oxidative stress: roles of poly(ADP-Ribose) polymerases and Ca(2+).
Authors:Wyrsch P, Blenn C, Bader J, Althaus FR,
Journal:Mol Cell Biol
PubMed ID:22751932
On the cellular level, oxidative stress may cause various responses, including autophagy and cell death. All of these outcomes involve disturbed Ca(2+) signaling. Here we show that the nuclear enzymes poly(ADP-ribose) polymerase 1 (PARP1) and PARP2 control cytosolic Ca(2+) shifts from extracellular and intracellular sources associated with autophagy or cell ... More
The Nef protein of human immunodeficiency virus is a broad-spectrum modulator of chemokine receptor cell surface levels that acts independently of classical motifs for receptor endocytosis and Galphai signaling.
Authors:Michel N, Ganter K, Venzke S, Bitzegeio J, Fackler OT, Keppler OT
Journal:Mol Biol Cell
PubMed ID:16775006
'Chemokine receptors (CKRs) are important physiological mediators of immune defense, inflammatory responses, and angiogenesis, and they have also been implicated in a number of viral disease processes. Here, we report that the Nef protein of human immunodeficiency virus (HIV) reduces cell surface levels of eight different members of the CC- ... More
Development and validation of a cell-based high-throughput screening assay for TRPM2 channel modulators.
Authors:Song Y, Buelow B, Perraud AL, Scharenberg AM,
Journal:J Biomol Screen
PubMed ID:18057180
'TRPM2 is a member of the transient receptor potential melastatin (TRPM)-related ion channel family. The activation of TRPM2 induced by oxidative/nitrosative stress leads to an increase in intracellular free Ca(2+). Although further progress in understanding TRPM2''s role in cell and organism physiology would be facilitated by isolation of compounds able ... More
Fc receptor-like 5 inhibits B cell activation via SHP-1 tyrosine phosphatase recruitment.
Authors:Haga CL, Ehrhardt GR, Boohaker RJ, Davis RS, Cooper MD
Journal:Proc Natl Acad Sci U S A
PubMed ID:17522256
'The Fc receptor-like protein 5 (FCRL5) on B cells has both an immunoreceptor tyrosine-based activation motif (ITAM)-like sequence and two consensus immunoreceptor tyrosine-based inhibitory motifs (ITIM) in its cytoplasmic region. To evaluate its signaling potential, we expressed constructs for chimeric molecules composed of the cytoplasmic region of FCRL5 and the ... More
Pertussis toxin signals through the TCR to initiate cross-desensitization of the chemokine receptor CXCR4.
Authors:Schneider OD, Weiss AA, Miller WE,
Journal:J Immunol
PubMed ID:19380820
Pertussis toxin (PTx) has been shown to exert a variety of effects on immune cells independent of its ability to ADP-ribosylate G proteins. Of these effects, the binding subunit of PTx (PTxB) has been shown to block signaling via the chemokine receptor CCR5, but the mechanism involved in this process ... More
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