Spectral unmixing: analysis of performance in the olfactory bulb in vivo.
AuthorsDucros M, Moreaux L, Bradley J, Tiret P, Griesbeck O, Charpak S,
JournalPLoS One
PubMed ID19198655
BACKGROUND: The generation of transgenic mice expressing combinations of fluorescent proteins has greatly aided the reporting of activity and identification of specific neuronal populations. Methods capable of separating multiple overlapping fluorescence emission spectra, deep in the living brain, with high sensitivity and temporal resolution are therefore required. Here, we investigate ... More