FocalCheck™ Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration - FAQs

What are the excitation/emission wavelengths for the beads in FocalCheck Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration (Cat. No. F36909)?

The spectral characteristics are as follows:

• A1: 505/515, 560/580, 660/680
• A2: 365/430 (core), 505/515 (ring)
• A3-5: 365/430, 505/515, 580/600, 660/680
• B1-5: 505/515

What type of coverslips are used for the FocalCheck Fluorescence Microscope Test Slide #1 (Cat. No. F36909)?

The coverslips are made of borosilicate glass (#1.5; ~0.17 mm thickness).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the microspheres from FocalCheck Fluorescence Microscope Test Slide #1 (Cat. No. F36909) in one plane?

No. Most of the 15 µm beads (Position A1 on the slide) would be within the narrowest focal plane, but they are not guaranteed to all be within the same plane.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.