Quality assessment of confocal microscopy slide-based systems: instability.
AuthorsZucker RM
JournalCytometry A
PubMed ID16807898
'BACKGROUND: All slide-based fluorescence cytometry detections systems basically include an excitation light source, intermediate optics, and a detection device (CCD or PMT). Occasionally, this equipment becomes unstable, generating unreliable and inferior data. METHODS: A number of tests have been devised to evaluate equipment performance and instability. The following four instability ... More
Reliability of confocal microscopy spectral imaging systems: use of multispectral beads.
AuthorsZucker RM, Rigby P, Clements I, Salmon W, Chua M,
JournalCytometry A
PubMed ID17266146
BACKGROUND: There is a need for a standardized, impartial calibration, and validation protocol on confocal spectral imaging (CSI) microscope systems. To achieve this goal, it is necessary to have testing tools to provide a reproducible way to evaluate instrument performance. METHODS: We evaluated fluorescent spectral beads (FocalCheck) from Molecular Probes/Invitrogen ... More
Snapshot hyperspectral imaging augments pixel dwell time and acquisition speeds over existing scanning systems, making it a powerful tool for fluorescence microscopy. While most snapshot systems contain fixed datacube parameters (x,y,?), our novel snapshot system, called the lenslet array tunable snapshot imaging spectrometer (LATIS), demonstrates tuning its average spectral resolution ... More