Wavelength and alignment tests for confocal spectral imaging systems.
AuthorsZucker RM, Lerner JM
JournalMicrosc Res Tech
PubMed ID16315239
'Confocal spectral imaging (CSI) microscope systems now on the market delineate multiple fluorescent proteins, labels, or dyes within biological specimens by performing spectral characterizations. However, we find that some CSI present inconsistent spectral profiles of reference spectra within a particular system as well as between related and unrelated instruments. We ... More
Quality assessment of confocal microscopy slide-based systems: instability.
AuthorsZucker RM
JournalCytometry A
PubMed ID16807898
'BACKGROUND: All slide-based fluorescence cytometry detections systems basically include an excitation light source, intermediate optics, and a detection device (CCD or PMT). Occasionally, this equipment becomes unstable, generating unreliable and inferior data. METHODS: A number of tests have been devised to evaluate equipment performance and instability. The following four instability ... More