Evaluation of methods for measuring cellular glutathione content using flow cytometry.
AuthorsHedley DW, Chow S
JournalCytometry
PubMed ID8026225
'The currently available flow cytometric stains for cellular glutathione were evaluated, examining the labelling of both human and rodent cell lines under various conditions of concentration, time, and temperature. Procedures were used that depleted glutathione (GSH) while having a minimal effect on other cellular sulphydryls in order to estimate linearity ... More
The location of fluorescence probes with charged groups in model membranes.
AuthorsKachel K, Asuncion-Punzalan E, London E
JournalBiochim Biophys Acta
PubMed ID9814853
'The location of commonly used charged fluorescent membrane probes in membranes was determined in order to: (1) investigate the relationship between the structure of hydrophobic molecules and their depth within membranes; and (2) aid interpretation of experiments in which these fluorescent probes are used to examine membrane structure. Membrane depth ... More
Fluorescence resonance energy transfer analysis of lipopolysaccharide in detergent micelles.
AuthorsWiström CA, Jones GM, Tobias PS, Sklar LA
JournalBiophys J
PubMed ID8789116
Bacterial endotoxins or lipopolysaccharides (LPS), cell wall components of gram-negative bacteria, are involved in septic shock. LPS consists of a lipid A tail attached to core and O-antigen polysaccharides, but little is known about the supramolecular structure of LPS in blood. We have developed an approach to locate donor and ... More
Use of four-colour flow cytometry to evaluate conjugate formation between human peripheral blood mononuclear cells and tumour target cells.
AuthorsKing MA, Radicchi-Mastroianni MA
JournalCytometry
PubMed ID8866221
A four-colour flow cytometry technique is described for the determination of the number and phenotype of conjugate-forming mononuclear effector cells from human blood. The discriminatory power of previously described techniques was improved by labelling the effector cells with antibodies that simultaneously identified natural killer (NK), T, and myeloid cells and ... More