Fluorescein Phalloidin, Green, 496/516 nm - FAQs

查看更多产品信息 Phalloidin Labeling Probes - FAQs (R415, A12379, A30104, B7474, A22282, A30105, A22281, A30106, A30107, A22286, P3457, F432, A22285, A22284, A22283, T7471, A22287, B3475, A34055, A12380, O7466, A12381, A34054)

3 个常见问题解答

我试着用鬼笔环肽结合F-actin标记我的石蜡切片，但是没有看到信号，为什么？

当细胞和组织经由二甲苯或丙酮之类的溶剂处理时（例如组织切片脱石蜡期间），它通过阻止鬼笔环肽结合的方式来影响F- actin。冰冻切片通常不使用有机溶剂洗涤，可以用来代替石蜡切片，也可以使用抗肌动蛋白抗体。

I want to label F-actin with phalloidin and image with transmission electron microscopy. What is the best way to go about it?

One way is to use a biotin-phalloidin conjugate, then label that with a colloidal gold conjugate of streptavidin. You will need to block endogenous biotin first. Another method is to label with fluorescein phalloidin, then with an anti-fluorescein antibody conjugated with colloidal gold.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?

When cells and tissues are treated with solvents such as xylene or acetone (for example during deparaffinization of tissue sections), it affects the F-actin in a way that prevents phalloidins from binding. Phalloidin may be used with cryosections, which are not typically washed with organic solvents, or anti-actin antibodies may be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.