Ultrasensitive detection of pathological prion protein aggregates by dual-color scanning for intensely fluorescent targets.
AuthorsBieschke J, Giese A, Schulz-Schaeffer W, Zerr I, Poser S, Eigen M, Kretzschmar H
JournalProc Natl Acad Sci U S A
PubMed ID10805803
'A definite diagnosis of prion diseases such as Creutzfeldt-Jakob disease (CJD) relies on the detection of pathological prion protein (PrP(Sc)). However, no test for PrP(Sc) in cerebrospinal fluid (CSF) has been available thus far. Based on a setup for confocal dual-color fluorescence correlation spectroscopy, a technique suitable for single molecule ... More
Calcium-mediated transductive systems and functionally active gap junctions in astrocyte-like GL15 cells.
AuthorsMariggio MA, Mazzoleni G, Pietrangelo T, Guarnieri S, Morabito C, Steimberg N, Fano G
JournalBMC Physiol
PubMed ID11384510
BACKGROUND: It has been proposed that GL15, a human cell line derived from glioblastoma multiforme, is a possible astroglial-like cell model, based on the presence of cytoplasmic glial fibrillary acidic protein. RESULTS: The aim of this work was to delineate the functional characteristics of GL15 cells using various experimental approaches, ... More