Interactions of histone H1 with phospholipids and comparison of its binding to giant liposomes and human leukemic T cells.
AuthorsZhao H, Bose S, Tuominen EK, Kinnunen PK,
JournalBiochemistry
PubMed ID15287747
Due to its net positive charge histone H1 readily associates with liposomes containing acidic phospholipids, such as phosphatidylserine (PS). Interestingly, circular dichroism reveals that while histone H1 in aqueous solutions appears as a random coil, its binding to liposomes containing PS is associated with a pronounced increase in alpha-helicity and ... More
Bacterial lipopolysaccharide can enter monocytes via two CD14-dependent pathways.
AuthorsKitchens RL, Wang P, Munford RS
JournalJ Immunol
PubMed ID9820530
Host recognition and disposal of LPS, an important Gram-negative bacterial signal molecule, may involve intracellular processes. We have therefore analyzed the initial pathways by which LPS, a natural ligand of glycosylphosphatidylinositol (GPI)-anchored CD14 (CD14-GPI), enters CD14-expressing THP-1 cells and normal human monocytes. Exposure of the cells to hypertonic medium obliterated ... More
Calcium-mediated transductive systems and functionally active gap junctions in astrocyte-like GL15 cells.
AuthorsMariggio MA, Mazzoleni G, Pietrangelo T, Guarnieri S, Morabito C, Steimberg N, Fano G
JournalBMC Physiol
PubMed ID11384510
BACKGROUND: It has been proposed that GL15, a human cell line derived from glioblastoma multiforme, is a possible astroglial-like cell model, based on the presence of cytoplasmic glial fibrillary acidic protein. RESULTS: The aim of this work was to delineate the functional characteristics of GL15 cells using various experimental approaches, ... More