Determination of progesterone and 17-hydroxyprogesterone by high performance liquid chromatography after pre-column derivatization with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionohydra zide.
AuthorsKatayama M, Nakane R, Matsuda Y, Kaneko S, Hara I, Sato H
JournalAnalyst
PubMed ID10396811
'Progesterone, 17-hydroxyprogesterone and four other 3-keto steroids were determined by high performance liquid chromatography with fluorescence detection. Each steroid was derivatized with 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene++ +-3- propionohydrazide (BODIPY FL hydrazide) and separated on a Wakosil 5C4 column with acetonitrile-water (7 + 3) as mobile phase. The limits of detection of progesterone, 17-hydroxyprogesterone, ... More
New method for high-performance liquid chromatographic separation and fluorescence detection of ginsenosides.
AuthorsShangguan D, Han H, Zhao R, Zhao Y, Xiong S, Liu G
JournalJ Chromatogr A
PubMed ID11261731
A novel pre-column derivatization method for the quantitative determination of ginsenosides by HPLC with fluorescence detection was established. The double bond at the C24-C25 position of ginsenoside was converted into an aldehyde group by means of ozonolysis. Then the aldehyde group reacts with FMOC-hydrazine forming the ginsenoside FMOC-hydrazone. The derivatized ... More
Determination of blood sugars by high pressure liquid chromatography with fluorescent detection.
AuthorsYuh YS, Chen JL, Chiang CH
JournalJ Pharm Biomed Anal
PubMed ID9547710
In this study, a high pressure liquid chromatography method with fluorescent detector was developed to analyze blood galactose, lactose and glucose simultaneously. Plasma sugars were prepared as fluorescent derivatives to react with FMOC-hydrazine (9-fluorenyl methyl chloroformate). A C18 reversed phase column and a fluorescent detector were used and run in ... More
Synthesis and application of Fmoc-hydrazine for the quantitative determination of saccharides by reversed-phase high-performance liquid chromatography in the low and subpicomole range.
AuthorsZhang RE, Cao YL, Hearn MW
JournalAnal Biochem
PubMed ID1888013
A new derivatization reagent, Fmoc-hydrazine, has been synthesized from the reaction of Fmoc-chloroformate with hydrazine as a precolumn fluorometric labeling reagent for reducing sugars such as glucose, galactose, mannose, fructose, fucose, ribose, xylose, arabinose, lactose, and maltose. The optimization of derivatization conditions was examined in detail. Using a reversed-phase high-performance ... More
Simple and sensitive multi-sugar-probe gut permeability test by high-performance liquid chromatography with fluorescence labelling.
AuthorsRooyakkers DR, van Eijk HM, Deutz NE
JournalJ Chromatogr A
PubMed ID8680601
Enteral intake of a mixture of inert, non-metabolic monosaccharide and disaccharide probes, followed by measurement of their urinary probe ratio, is a well known method to investigate gut permeability. However, most applications lack sensitivity, thus a large amount of especially the disaccharide lactulose has to be ingested. This may cause ... More