Acid-induced conformational changes in phosphoglucose isomerase result in its increased cell surface association and deposition on fibronectin fibrils.
AuthorsAmraei M, Jia Z, Reboul P, Nabi IR
JournalJ Biol Chem
PubMed ID12888574
'Phosphoglucose isomerase (PGI) is a glycolytic enzyme that exhibits extracellular cytokine activity as autocrine motility factor, neuroleukin, and maturation factor and that has been recently implicated as an autoantigen in rheumatoid arthritis. In contrast to its receptor-mediated endocytosis at neutral pH, addition of 25 microg/ml of either Alexa 568- or ... More
Novel role for galectin-8 protein as mediator of coagulation factor V endocytosis by megakaryocytes.
AuthorsZappelli C, van der Zwaan C, Thijssen-Timmer DC, Mertens K, Meijer AB,
JournalJ Biol Chem
PubMed ID22267735
'Galectin-8 (Gal8) interacts with ß-galactoside-containing glycoproteins and has recently been implicated to play a role in platelet activation. It has been suggested that Gal8 may also interact with platelet coagulation factor V (FV). This indispensable cofactor is stored in a-granules of platelets via a poorly understood endocytic mechanism that only ... More
Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.
'Amelogenin is an extracellular matrix protein secreted by ameloblasts and is a major component of enamel matrix. Recently, in addition to their role in enamel formation, the biological activity of enamel proteins in the process of cell differentiation has recently become widely appreciated. In this study, we examined the biological ... More
Surface-functionalized nanoparticle library yields probes for apoptotic cells.
AuthorsSchellenberger EA, Reynolds F, Weissleder R, Josephson L
JournalChembiochem
PubMed ID14997519
'We have developed techniques for the efficient synthesis and screening of small libraries of surface-functionalized nanoparticles for the recognition of specific types of cells. To illustrate this concept we describe the development of a nanoparticle that preferentially recognizes apoptotic Jurkat cells in a manner similar to the apoptosis-recognizing protein annexin ... More
Heparin-induced thrombocytopenia: in vitro studies on the interaction of dabigatran, rivaroxaban, and low-sulfated heparin, with platelet factor 4 and anti-PF4/heparin antibodies.
AuthorsKrauel K, Hackbarth C, Fürll B, Greinacher A,
JournalBlood
PubMed ID22049520
'Heparin is a widely used anticoagulant. Because of its negative charge, it forms complexes with positively charged platelet factor 4 (PF4). This can induce anti-PF4/heparin IgG Abs. Resulting immune complexes activate platelets, leading to the prothrombotic adverse drug reaction heparin-induced thrombocytopenia (HIT). HIT requires treatment with alternative anticoagulants. Approved for ... More
Double staining technique using a combination of indirect and direct immunofluorescence with monoclonal antibodies.
AuthorsKupper H, Storz H
JournalActa Histochem
PubMed ID2425537
A simple method for double staining by immunofluorescence is described. If for double staining using monoclonal antibodies of the same species only one antibody is conjugated with FITC or TRITC, a combination of indirect and direct immunofluorescence is possible. For cell staining the following incubation steps are carried out: Monoclonal ... More
Trimeric autotransporter DsrA is a major mediator of fibrinogen binding in Haemophilus ducreyi.
AuthorsFusco WG, Elkins C, Leduc I,
Journal
PubMed ID24042118
Haemophilus ducreyi is the etiologic agent of the sexually transmitted genital ulcer disease chancroid. In both natural and experimental chancroid, H. ducreyi colocalizes with fibrin at the base of the ulcer. Fibrin is obtained by cleavage of the serum glycoprotein fibrinogen (Fg) by thrombin to initiate formation of the blood ... More
Modification of an exposed loop in the C1 domain reduces immune responses to factor VIII in hemophilia A mice.
AuthorsWroblewska A, van Haren SD, Herczenik E, Kaijen P, Ruminska A, Jin SY, Zheng XL, van den Biggelaar M, ten Brinke A, Meijer AB, Voorberg J,
JournalBlood
PubMed ID22498747
Development of neutralizing Abs to blood coagulation factor VIII (FVIII) provides a major complication in hemophilia care. In this study we explored whether modulation of the uptake of FVIII by APCs can reduce its intrinsic immunogenicity. Endocytosis of FVIII by professional APCs is significantly blocked by mAb KM33, directed toward ... More
Phagocytosis by macrophages and endothelial cells inhibits procoagulant and fibrinolytic activity of acute promyelocytic leukemia cells.
AuthorsXie R, Gao C, Li W, Zhu J, Novakovic V, Wang J, Ma R, Zhou J, Gilbert GE, Shi J,
JournalBlood
PubMed ID22138513
The coagulopathy of acute promyelocytic leukemia (APL) is mainly related to procoagulant substances and fibrinolytic activators of APL blasts, but the fate of these leukemic cells is unknown. The aim of this study was to investigate the removal of APL blasts by macrophages and endothelial cells in vitro and consequent ... More
Structural analysis of a bacterial exo-a-D-N-acetylglucosaminidase in complex with an unusual disaccharide found in class III mucin.
AuthorsFicko-Blean E, Boraston AB,
JournalGlycobiology
PubMed ID22090394
CpGH89 is a family 89 glycoside hydrolase with exo-a-D-N-acetylglucosaminidase activity that is produced by the human and animal pathogen Clostridium perfringens. This enzyme is active on the a-D-GlcpNAc-(1 ? 4)-D-Galp motif that is displayed on the class III mucins within the gastric mucosa. Other members of this enzyme family, such ... More
Gadd45 beta mediates the NF-kappa B suppression of JNK signalling by targeting MKK7/JNKK2.
AuthorsPapa S, Zazzeroni F, Bubici C, Jayawardena S, Alvarez K, Matsuda S, Nguyen DU, Pham CG, Nelsbach AH, Melis T, De Smaele E, Tang WJ, D'Adamio L, Franzoso G
JournalNat Cell Biol
PubMed ID14743220
NF-kappa B/Rel transcription factors control apoptosis, also known as programmed cell death. This control is crucial for oncogenesis, cancer chemo-resistance and for antagonizing tumour necrosis factor alpha (TNFalpha)-induced killing. With regard to TNFalpha, the anti-apoptotic activity of NF-kappa B involves suppression of the c-Jun N-terminal kinase (JNK) cascade. Using an ... More