Quantitative nonradioactive CAT assays using fluorescent BODIPY 1-deoxychloramphenicol substrates.
AuthorsLefevre CK, Singer VL, Kang HC, Haugland RP
JournalBiotechniques
PubMed ID7495564
'We report the development of fluorescent BODIPY 1-deoxychloramphenicol substrates for chloramphenicol acetyltransferase (CAT). These substrates not only simplify and improve quantitation of CAT activity but also extend the linear range of detection. Because the 1-deoxychloramphenicol derivatives have only one acetylation site, the enzyme reaction creates only one product, whereas chloramphenicol ... More