'Tight junctions between intestinal epithelial cells prevent ingress of luminal macromolecules and bacteria and protect against inflammation and infection. During stress and inflammation, mast cells mediate increased mucosal permeability by unknown mechanisms. We hypothesized that mast cell tryptase cleaves protease-activated receptor 2 (PAR2) on colonocytes to increase paracellular permeability. Colonocytes ... More
A practical guide to the preparation of Ca2+ buffers.
AuthorsBers DM, Patton CW, Nuccitelli R
JournalMethods Cell Biol
PubMed ID8201981
Calcium imaging of cortical neurons using Fura-2 AM.
AuthorsBarreto-Chang OL, Dolmetsch RE,
JournalJ Vis Exp
PubMed ID19229178
Calcium imaging is a common technique that is useful for measuring calcium signals in cultured cells. Calcium imaging techniques take advantage of calcium indicator dyes, which are BAPTA-based organic molecules that change their spectral properties in response to the binding of Ca2+ ions. Calcium indicator dyes fall into two categories, ... More
TRPM7 activation potentiates SOCE in enamel cells but requires ORAI.
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P2Y receptor regulation of sodium transport in human mammary epithelial cells.
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Motile ghosts of the halophilic archaeon, Haloferax volcanii.
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K(Ca)3.1 channels facilitate K+ secretion or Na+ absorption depending on apical or basolateral P2Y receptor stimulation.
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Intracellular delivery and calcium transients generated in sonoporation facilitated by microbubbles.
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Involvement of endoplasmic reticulum stress in a novel Classic Galactosemia model.
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Fibrillin-1 directly regulates osteoclast formation and function by a dual mechanism.
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Effect of Calstabin1 depletion on calcium transients and energy utilization in muscle fibers and treatment opportunities with RyR1 stabilizers.
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Alpha-synuclein aggregates activate calcium pump SERCA leading to calcium dysregulation.