Intracellular Ca2+ and pacemaking within the rabbit sinoatrial node: heterogeneity of role and control.
AuthorsLancaster MK, Jones SA, Harrison SM, Boyett MR,
JournalJ Physiol
PubMed ID14724216
'Recent studies have proposed that release of calcium from the sarcoplasmic reticulum (SR) modulates the spontaneous activity of the sinoatrial node (SAN). Previously we have shown that several calcium regulatory proteins are expressed at a lower level in the centre of the SAN compared with the periphery. Such differences may ... More
Quality assessment of confocal microscopy slide based systems: performance.
AuthorsZucker RM
JournalCytometry A
PubMed ID16807897
'BACKGROUND: All fluorescence slide-based cytometry detections systems basically include the following components: (1) an excitation light source, (2) intermediate optics, and (3) a detection device consisting of a CCD camera or a PMT. The optical principles employed is slide-based systems are similar to those of confocal microscopes (CLSM). METHODS: The ... More
Targeting of U2AF65 to sites of active splicing in the nucleus.
AuthorsGama-Carvalho M, Krauss RD, Chiang L, Valcárcel J, Green MR, Carmo-Fonseca M
JournalJ Cell Biol
PubMed ID9166400
U2AF65 is an essential splicing factor that promotes binding of U2 small nuclear (sn)RNP at the pre-mRNA branchpoint. Here we describe a novel monoclonal antibody that reacts specifically with U2AF65. Using this antibody, we show that U2AF65 is diffusely distributed in the nucleoplasm with additional concentration in nuclear speckles, which ... More