Targeting of U2AF65 to sites of active splicing in the nucleus.
AuthorsGama-Carvalho M, Krauss RD, Chiang L, Valcárcel J, Green MR, Carmo-Fonseca M
JournalJ Cell Biol
PubMed ID9166400
U2AF65 is an essential splicing factor that promotes binding of U2 small nuclear (sn)RNP at the pre-mRNA branchpoint. Here we describe a novel monoclonal antibody that reacts specifically with U2AF65. Using this antibody, we show that U2AF65 is diffusely distributed in the nucleoplasm with additional concentration in nuclear speckles, which ... More
Flexible Multi-Beam Light-Sheet Fluorescence Microscope for Live Imaging Without Striping Artifacts.
AuthorsSancataldo G, Gavryusev V, de Vito G, Turrini L, Locatelli M, Fornetto C, Tiso N, Vanzi F, Silvestri L, Pavone FS
JournalFront Neuroanat
PubMed ID30800060
The development of light-sheet fluorescence microscopy (LSFM) has greatly expanded the experimental capabilities in many biological and biomedical research fields, enabling for example live studies of murine and zebrafish neural activity or of cell growth and division. The key feature of the method is the selective illumination of a sample ... More