New fluorescent probes for protein kinase C. Synthesis, characterization, and application.
AuthorsChen CS, Poenie M
JournalJ Biol Chem
PubMed ID8340406
Fluorescent derivatives of the bisindolylmaleimide inhibitors of protein kinase C (PKC) were synthesized and tested with respect to their inhibitory potency, specificity, and usefulness as fluorescent cytological stains for PKC. Several of the fluorescent bisindolylmaleimide derivatives (fim-1, fim-2, and rim-1) acted as ATP-competitive catalytic site inhibitors and retained much of ... More