Nanoparticles as fluorescence labels: is size all that matters?
AuthorsSwift JL, Cramb DT,
JournalBiophys J
PubMed ID18390610
'Fluorescent labels are often used in bioassays as a means to detect and characterize ligand-receptor binding. This is due in part to the inherently high sensitivity of fluorescence-based technology and the relative accessibility of the technique. There is often little concern raised as to whether or not the fluorescent label ... More
Three-dimensional, single-molecule fluorescence imaging beyond the diffraction limit by using a double-helix point spread function.
AuthorsPavani SR, Thompson MA, Biteen JS, Lord SJ, Liu N, Twieg RJ, Piestun R, Moerner WE,
JournalProc Natl Acad Sci U S A
PubMed ID19211795
We demonstrate single-molecule fluorescence imaging beyond the optical diffraction limit in 3 dimensions with a wide-field microscope that exhibits a double-helix point spread function (DH-PSF). The DH-PSF design features high and uniform Fisher information and has 2 dominant lobes in the image plane whose angular orientation rotates with the axial ... More