AuthorsHinz M, Gura S, Nitzan B, Margel S, Seliger H
JournalJ Biotechnol
PubMed ID11257537
'Different kinds of particles were investigated for their potential use as supports for exonucleolytic sequence analysis. Composite beads composed of an unreactive polystyrene "core" and a "shell" of functionalized silica nanoparticles were found to best fulfill the various prerequisites. The biotin/streptavidin system was used for attachment of DNA to composite ... More
Comparison of detection and signal amplification methods for DNA microarrays.
AuthorsVora GJ, Meador CE, Anderson GP, Taitt CR,
JournalMol Cell Probes
PubMed ID18675897
One of the factors limiting the use of DNA microarray technology for the detection of pathogenic organisms from clinical and environmental matrices has been inadequate assay sensitivity. To assess the effectiveness of post-hybridization secondary detection steps to enhance the sensitivity of DNA microarray-based pathogen detection, we evaluated a panel of ... More
The strategy of T cell antigen-presenting cell encounter in antigen-draining lymph nodes revealed by imaging of initial T cell activation.
AuthorsBajénoff M, Granjeaud S, Guerder S
JournalJ Exp Med
PubMed ID12953093
The development of an immune response critically relies on the encounter of rare antigen (Ag)-specific T cells with dendritic cells (DCs) presenting the relevant Ag. How two rare cells find each other in the midst of irrelevant other cells in lymph nodes (LNs) is unknown. Here we show that initial ... More