High sensitivity immunoassays using particulate fluorescent labels.
AuthorsHall M, Kazakova I, Yao YM
JournalAnal Biochem
PubMed ID10415085
'The use of polystyrene fluorescent microspheres as sensitive labels in direct-detection (not enzymatically amplified) heterogeneous equilibrium "sandwich" immunoassays in 96-well plates is described. With mouse IgG as a model antigen, a fluorescent particulate label is more sensitive than a corresponding soluble reporter. The limit of detection of mouse IgG in ... More