FluoSpheres™ Streptavidin-Labeled Microspheres, 0.04 μm, yellow-green fluorescent (505/515), 0.5% solids
FluoSpheres™ Streptavidin-Labeled Microspheres, 0.04 μm, yellow-green fluorescent (505/515), 0.5% solids
Citations & References (4)
Invitrogen™

FluoSpheres™ Streptavidin-Labeled Microspheres, 0.04 μm, yellow-green fluorescent (505/515), 0.5% solids

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed fromRead more
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Catalog NumberQuantity
F8780
also known as F-8780
0.4 mL
Catalog number F8780
also known as F-8780
Price (CNY)
7,868.00
Each
Add to cart
Quantity:
0.4 mL
Price (CNY)
7,868.00
Each
Add to cart

Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes™ FluoSpheres™ beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy. Different surface modifications are available to facilitate the coupling of various molecules and proteins to the surface of the bead.

FluoSpheres™ Microsphere Specifications

  • Label (Ex/Em): Yellow-green fluorescent (505/515 nm)
  • Nominal bead diameter: 0.04 μm
  • Surface modification: Streptavidin
  • Solids: 0.5%

Characteristics of the Various FluoSpheres™ Coupling Surfaces

  • Carboxylate-modified FluoSpheres™ beads have a high density of pendent carboxylic acids on their surface, making them suitable for covalent coupling of proteins and other amine-containing biomolecules using water-soluble carbodiimide reagents such as EDAC
  • Sulfate FluoSpheres™ beads are relatively hydrophobic particles that will passively and nearly irreversibly adsorb almost any protein, including albumin, IgG, avidin, and streptavidin
  • Aldehyde-sulfate FluoSpheres™ beads have surface aldehyde groups added, designed to react with proteins and other amines under very mild conditions.
  • Amine-modified FluoSpheres™ beads can be coupled to a wide variety of amine-reactive molecules, including the succinimidyl esters and isothiocyanates of haptens and drugs or the carboxylic acids of proteins, using a water-soluble carbodiimide

Key Applications of Microspheres

  • Instrument calibration (flow cytometry, microscopy, HTS, HCS)
  • Flow testing (microfluidics, blood flow, water flow, and air flow)
  • Cell biology tracers (cell differentiation and cell tracing)
  • Immunoassays (agglutination tests, ELISA, particle capture, and contrast reagents)

Choices for FluoSpheres™ Fluorescent Microspheres

Among our complete offering of fluorescent microspheres products, you'll find beads with these variations:

  • Ten fluorescent colors
  • Ten nominal bead diameters: 0.02 μm, 0.04 μm, 0.1 μm, 0.2 μm, 0.5 μm, 1.0 μm, 2.0 μm, 4.0 μm, 10.0 μm, and 15.0 μm
  • Four surface modifications for protein coupling: carboxylate, sulfate, aldehyde-sulfate, amine
  • Microspheres that are additionally precoupled with streptavidin, NeutrAvidin, biotin, europium, and platinum

Choices for Unstained Microspheres

We also offer hundreds of choices for UltraClean™ surfactant-free microspheres for research and commercial applications.

We'll Make a Custom Microsphere Product for You

We will prepare custom orders upon request. For example, FluoSpheres™ beads can be prepared with intensities that are lower than those of our regular selection, a desirable feature in some multicolor applications. Our custom conjugation service is efficient and confidential, and we guarantee the quality of our work. We are ISO 13485:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product LineFLUOSPHERES
Quantity0.4 mL
Shipping ConditionRoom Temperature
Surface ModificationOther Modification or Label
ColorYellow-Green
Diameter (Metric)0.04 μm
MaterialPolystyrene
Product TypeStreptavidin Microspheres
Unit SizeEach
Contents & Storage
Store in refrigerator (2°C to 8°C) and protect from light.

Frequently asked questions (FAQs)

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

I accidentally froze my microspheres; can I still use them?

Even brief freezing can cause irreversible aggregation and potential distortion of the bead shape. You should not use these microspheres.

Find additional tips, troubleshooting help, and resources within our Microspheres Support Center.

My protein-coated microspheres appear to be non-specifically binding in my experimental system. Do you have a product that can help reduce these non-specific interactions?

Non-specific binding can often be relieved by a blocking solution, but microspheres seem to require a stronger blocking solution than those most commonly commercially available. Hence, we've developed the BlockAid Blocking Solution (Cat. No. B10710). This reagent is a protein-based blocking solution designed for use with FluoSpheres microspheres and TransFluoSpheres microspheres conjugated to biotin, streptavidin, NeutrAvidin biotin-binding protein, or other proteins. The BlockAid Blocking Solution has proven useful for reducing the nonspecific binding of protein-coated or other macromolecule-coated microspheres in a wide variety of flow cytometry, microscopy, and microarray applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all FluoSpheres™ Streptavidin-Labeled Microspheres, 0.04 μm, yellow-green fluorescent (505/515), 0.5% solids FAQs

Citations & References (4)

Citations & References
Abstract
Nanoparticles as fluorescence labels: is size all that matters?
Authors:Swift JL, Cramb DT,
Journal:Biophys J
PubMed ID:18390610
'Fluorescent labels are often used in bioassays as a means to detect and characterize ligand-receptor binding. This is due in part to the inherently high sensitivity of fluorescence-based technology and the relative accessibility of the technique. There is often little concern raised as to whether or not the fluorescent label ... More
Green- and red-fluorescent nanospheres for the detection of cell surface receptors by flow cytometry.
Authors:Bhalgat MK, Haugland RP, Pollack JS, Swan S, Haugland RP
Journal:J Immunol Methods
PubMed ID:9831388
'Fluorescent probes serve as sensitive tools for obtaining structural and functional information in cellular systems. In spite of the high sensitivity provided by fluorescent reagents, cell surface receptors expressed in low numbers often escape detection with commonly used fluorescent probes. R-Phycoerythrin (R-PE), a molecule with a very high quantum yield, ... More
A role for transferrin receptor in triggering apoptosis when targeted with gambogic acid.
Authors:Kasibhatla S, Jessen KA, Maliartchouk S, Wang JY, English NM, Drewe J, Qiu L, Archer SP, Ponce AE, Sirisoma N, Jiang S, Zhang HZ, Gehlsen KR, Cai SX, Green DR, Tseng B
Journal:Proc Natl Acad Sci U S A
PubMed ID:16103367
Transferrin receptor (TfR) has been shown to be significantly overexpressed in different types of cancers. We discovered TfR as a target for gambogic acid (GA), used in traditional Chinese medicine and a previously undiscovered link between TfR and the rapid activation of apoptosis. The binding site of GA on TfR ... More
Engineering novel cell surface receptors for virus-mediated gene transfer.
Authors:Lee JH, Baker TJ, Mahal LK, Zabner J, Bertozzi CR, Wiemer DF, Welsh MJ
Journal:J Biol Chem
PubMed ID:10419507
The absence of viral receptors is a major barrier to efficient gene transfer in many cells. To overcome this barrier, we developed an artificial receptor based on expression of a novel sugar. We fed cells an unnatural monosaccharide, a modified mannosamine that replaced the acetyl group with a levulinate group ... More