Influenza hemagglutinins outside of the contact zone are necessary for fusion pore expansion.
AuthorsLeikina E, Mittal A, Cho MS, Melikov K, Kozlov MM, Chernomordik LV
JournalJ Biol Chem
PubMed ID15078874
Current models for membrane fusion in diverse biological processes are focused on the local action of fusion proteins present in the contact zone where the proteins anchored in one membrane might interact directly with the other membrane. Are the fusion proteins outside of the contact zone just bystanders? Here we ... More
Preparation of DNA-modified nanoparticles and preliminary study for colorimetric SNP analysis using their selective aggregations.
AuthorsIhara T, Tanaka S, Chikaura Y, Jyo A
JournalNucleic Acids Res
PubMed ID15254253
DNA-modified nanospheres were prepared by anchoring amino-terminated oligodeoxynucleotides (ODNs) with carboxylates onto a colored polystyrene sphere surface through amido bonds. About 220 ODN molecules were immobilized onto a nanosphere 40 nm in diameter. Preliminary studies using the microspheres with 1 microm diameter reveal that the specificity of hybridization was retained ... More