Nano-sized fluorescent particles as new tracers for sentinel node detection: experimental model for decision of appropriate size and wavelength.
AuthorsNakajima M, Takeda M, Kobayashi M, Suzuki S, Ohuchi N
JournalCancer Sci
PubMed ID15958058
'The concepts of made-to-order and low-invasiveness medicines are becoming widely accepted. A treatment for cancer, with minimum invasive surgery and without lymph nodes dissection based on sentinel lymph node (SN) navigation surgery, would adhere to these concepts. Dyes and/or radioisotopes are employed for SN detection in standard methods, however, each ... More
A novel method to allow noninvasive, longitudinal imaging of the murine immune system in vivo.
AuthorsGibson VB, Benson RA, Bryson KJ, McInnes IB, Rush CM, Grassia G, Maffia P, Jenkinson EJ, White AJ, Anderson G, Brewer JM, Garside P,
JournalBlood
PubMed ID22271449
'In vivo imaging has revolutionized understanding of the spatiotemporal complexity that subserves the generation of successful effector and regulatory immune responses. Until now, invasive surgery has been required for microscopic access to lymph nodes (LNs), making repeated imaging of the same animal impractical and potentially affecting lymphocyte behavior. To allow ... More
Live-cell superresolution imaging by pulsed STED two-photon excitation microscopy.
AuthorsTakasaki KT, Ding JB, Sabatini BL,
JournalBiophys J
PubMed ID23442955
Two-photon laser scanning microscopy (2PLSM) allows fluorescence imaging in thick biological samples where absorption and scattering typically degrade resolution and signal collection of one-photon imaging approaches. The spatial resolution of conventional 2PLSM is limited by diffraction, and the near-infrared wavelengths used for excitation in 2PLSM preclude the accurate imaging of ... More