Distribution of retrogradely transported fluorescent latex microspheres in rat lumbosacral ventral root axons following peripheral crush injury: a light and electron microscopic study.
AuthorsPersson HG, Gatzinsky KP
JournalBrain Res
PubMed ID8118679
'The retrograde axonal transport of fluorescent latex microspheres, which are tracers extensively used for studying the neuronal connectivity in the CNS, was investigated in large myelinated lumbosacral ventral root nerve fibres of adult rats following peripheral crush injury. After crushing the sciatic nerve, a suspension of 30 nm red-fluorescent latex ... More
Identification of catecholaminergic inputs to and outputs from aromatase-containing brain areas of the Japanese quail by tract tracing combined with tyrosine hydroxylase immunocytochemistry.
AuthorsBalthazart J, Absil P
JournalJ Comp Neurol
PubMed ID9183702
'In the quail brain, aromatase-immunoreactive (ARO-ir) neurons located in the medial preoptic nucleus (POM) and caudal paleostriatum ventrale/nucleus accumbens/nucleus striae terminalis complex (PVT/nAc/nST) receive catecholaminergic inputs identified by the presence of tyrosine hydroxylase-immunoreactive (TH-ir) fibers and punctate structures. The origin of these inputs was analyzed by retrograde tracing with cholera ... More
The origins of catecholaminergic innervation in the rostral ventromedial medulla oblongata of the rat.
AuthorsTanaka M, Matsumoto Y, Murakami T, Hisa Y, Ibata Y
JournalNeurosci Lett
PubMed ID8710209
'The localization of catecholaminergic neuronal cell bodies which project to the rostral ventromedial medulla oblongata (RVM) were investigated by the combined technique with dopamine beta-hydroxylase (DBH), phenylethanolamine N-methyltransferase (PNMT) or tyrosine hydroxylase (TH) immunocytochemistry and retrograde neuronal tracing method using fluorescent latex microspheres (FluoSpheres) injected into the center of the ... More
Optimized localization analysis for single-molecule tracking and super-resolution microscopy.
AuthorsMortensen KI, Churchman LS, Spudich JA, Flyvbjerg H,
JournalNat Methods
PubMed ID20364147
'We optimally localized isolated fluorescent beads and molecules imaged as diffraction-limited spots, determined the orientation of molecules and present reliable formulas for the precision of various localization methods. Both theory and experimental data showed that unweighted least-squares fitting of a Gaussian squanders one-third of the available information, a popular formula ... More
T-type channels control the opioidergic descending analgesia at the low threshold-spiking GABAergic neurons in the periaqueductal gray.
AuthorsPark C, Kim JH, Yoon BE, Choi EJ, Lee CJ, Shin HS,
JournalProc Natl Acad Sci U S A
PubMed ID20682748
'Endogenous opioids generate analgesic signals in the periaqueductal gray (PAG). However, because cell types in the PAG are difficult to identify, its neuronal mechanism has remained poorly understood. To address this issue, we characterized PAG neurons by their electrical properties using differentially labeled GABAergic and output neurons in the PAG. ... More
Demonstration of cholinergic ganglion cells in rat retina: expression of an alternative splice variant of choline acetyltransferase.
AuthorsYasuhara O, Tooyama I, Aimi Y, Bellier JP, Hisano T, Matsuo A, Park M, Kimura H
JournalJ Neurosci
PubMed ID12684474
'Acetylcholine acts as a neurotransmitter in the retina. Although previous physiological studies have indicated that some retinal ganglion cells may be cholinergic, several immunohistochemical studies using antibodies to choline acetyltransferase (ChAT) have stained only amacrine cells but not ganglion cells. Recently, we identified a splice variant of ChAT mRNA, lacking ... More
Preparation of DNA-modified nanoparticles and preliminary study for colorimetric SNP analysis using their selective aggregations.
AuthorsIhara T, Tanaka S, Chikaura Y, Jyo A
JournalNucleic Acids Res
PubMed ID15254253
DNA-modified nanospheres were prepared by anchoring amino-terminated oligodeoxynucleotides (ODNs) with carboxylates onto a colored polystyrene sphere surface through amido bonds. About 220 ODN molecules were immobilized onto a nanosphere 40 nm in diameter. Preliminary studies using the microspheres with 1 microm diameter reveal that the specificity of hybridization was retained ... More
Fluorescent labeling of rat auditory brainstem circuits for synaptic and electrophysiological studies.
AuthorsMaruyama A, Ohmori H
JournalJ Neurosci Methods
PubMed ID16246426
We visualized neurons with fluorescent agents, both retrogradely and anterogradely, to identify the input and output neuronal pathways in the rat auditory system. Output neurons of dorsal cochlear nucleus (DCN) were labeled retrogradely by injecting fluorescent microspheres into the inferior colliculus. Electrical recordings were made from the labeled fusiform cells ... More
In situ fluorescent protein imaging with metal film-enhanced total internal reflection microscopy.
AuthorsBurghardt TP, Charlesworth JE, Halstead MF, Tarara JE, Ajtai K
JournalBiophys J
PubMed ID16565065
Fluorescence detection of single molecules provides a means to investigate protein dynamics minus ambiguities introduced by ensemble averages of unsynchronized protein movement or of protein movement mimicking a local symmetry. For proteins in a biological assembly, taking advantage of the single molecule approach could require single protein isolation from within ... More