Cyclic changes in keratocyte speed and traction stress arise from Ca2+-dependent regulation of cell adhesiveness.
AuthorsDoyle AD, Lee J
JournalJ Cell Sci
PubMed ID15632107
The activation of stretch-activated calcium channels (SACs) in keratocytes can induce spatially coordinated increases in traction stress that promote protrusion at the cell front, while simultaneously inducing retraction at the rear. To investigate how this occurs, we correlated calcium-induced changes in traction stress with alterations in cell speed and shape. ... More
Angiogenesis monitored by perfusion with a space-filling microbead suspension.
AuthorsSpringer ML, Ip TK, Blau HM
JournalMol Ther
PubMed ID10933915
Numerous laboratories are focusing efforts on delivering gene products to induce or prevent the development of new blood vessels in adults, with the hope of rescuing ischemic tissues, circumventing cardiac bypass surgery, or inhibiting tumor growth. Current approaches to the assessment of vascular continuity involve the introduction of either dyes ... More
Myoblast-mediated gene transfer for therapeutic angiogenesis.
AuthorsBanfi A, Springer ML, Blau HM,
JournalMethods Enzymol
PubMed ID11883066
Introduction Therapeutic angiogenesis has emerged in the past few years as one of the most promising applications of gene therapy. The goal is to deliver growth factors that control the formation of new vasculature to ischemic tissues in order to restore the blood supply that has been pathologically disrupted or ... More
Isolation of serotype-specific antibodies against dengue virus non-structural protein 1 using phage display and application in a multiplexed serotyping assay.
AuthorsLebani K, Jones ML, Watterson D, Ranzoni A, Traves RJ, Young PR, Mahler SM
JournalPLoS One
PubMed ID28683141
The multidimensional nature of dengue virus (DENV) infections, which can be caused by four distinct serotypes of the virus, complicates the sensitivity of assays designed for the diagnosis of infection. Different viral markers can be optimally detected at different stages of infection. Of particular clinical importance is the early identification ... More