Single-cell Raman and fluorescence microscopy reveal the association of lipid bodies with phagosomes in leukocytes.
Authorsvan Manen HJ, Kraan YM, Roos D, Otto C,
JournalProc Natl Acad Sci U S A
PubMed ID16002471
Cellular imaging techniques based on vibrational spectroscopy have become powerful tools in cell biology because the molecular composition of subcellular compartments can be visualized without the need for labeling. Using high-resolution, nonresonant confocal Raman microscopy on individual cells, we demonstrate here that lipid bodies (LBs) rich in arachidonate as revealed ... More
Counting dendritic spines in brain tissue slices by image correlation spectroscopy analysis.
AuthorsWiseman PW, Capani F, Squier JA, Martone ME
JournalJ Microsc
PubMed ID11879432
Growth of new micrometre sized projections called dendritic spines in neurones has been linked to the encoding of long-term memories in vertebrates. Numerous studies have been carried out at both the light and electron microscopy level to quantify dendritic spine densities in brain tissue in laboratory animals. Currently, such efforts ... More
Influenza hemagglutinins outside of the contact zone are necessary for fusion pore expansion.
AuthorsLeikina E, Mittal A, Cho MS, Melikov K, Kozlov MM, Chernomordik LV
JournalJ Biol Chem
PubMed ID15078874
Current models for membrane fusion in diverse biological processes are focused on the local action of fusion proteins present in the contact zone where the proteins anchored in one membrane might interact directly with the other membrane. Are the fusion proteins outside of the contact zone just bystanders? Here we ... More