Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network.
AuthorsPuertollano R, van der Wel NN, Greene LE, Eisenberg E, Peters PJ, Bonifacino JS
JournalMol Biol Cell
PubMed ID12686608
'Sorting of transmembrane proteins and their ligands at various compartments of the endocytic and secretory pathways is mediated by selective incorporation into clathrin-coated intermediates. Previous morphological and biochemical studies have shown that these clathrin-coated intermediates consist of spherical vesicles with a diameter of 60-100 nm. Herein, we report the use ... More
Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy.
AuthorsKulkarni RP, Wu DD, Davis ME, Fraser SE
JournalProc Natl Acad Sci U S A
PubMed ID15897455
'Quantitatively understanding how nonviral gene delivery vectors (polyplexes) are transported inside cells is essential before they can be optimized for gene therapy and medical applications. In this study, we used spatio-temporal image correlation spectroscopy (ICS) to follow polymer-nucleic acid particles (polyplexes) of various sizes and analyze their diffusive-like and flow ... More
Effects of atrial natriuretic peptide on phagocytosis and respiratory burst in murine macrophages.
AuthorsVollmar AM, Förster R, Schulz R
JournalEur J Pharmacol
PubMed ID9042602
'Atrial natriuretic peptide (ANP) is known to affect cardiovascular physiology displaying both hormone- and neurotransmitter characteristics. However, there is increasing evidence that ANP possesses additional biological activities referring to the immune system. To further strengthen this hypothesis the effect of ANP on two major functions of macrophages, i.e., phagocytosis and ... More
Precise microinjection into skin using hollow microneedles.
AuthorsWang PM, Cornwell M, Hill J, Prausnitz MR
JournalJ Invest Dermatol
PubMed ID16484988
'Hollow needles of micron dimensions have previously been fabricated and envisioned for use with transdermal patches or infusion pumps to achieve painless delivery of drugs to the skin for local and systemic effects without the need for hypodermic needles. However, little work has been carried out to identify methods to ... More
Why molecules move along a temperature gradient.
AuthorsDuhr S, Braun D
JournalProc Natl Acad Sci U S A
PubMed ID17164337
'Molecules drift along temperature gradients, an effect called thermophoresis, the Soret effect, or thermodiffusion. In liquids, its theoretical foundation is the subject of a long-standing debate. By using an all-optical microfluidic fluorescence method, we present experimental results for DNA and polystyrene beads over a large range of particle sizes, salt ... More
Rho is involved in superoxide formation during phagocytosis of opsonized zymosans.
AuthorsKim JS, Diebold BA, Kim JI, Kim J, Lee JY, Park JB
JournalJ Biol Chem
PubMed ID14970220
Phagocytosis is accompanied by the production of superoxide by the NADPH oxidase complex, for which GTP-bound Rac is essential. We wanted to determine whether Rho is also involved in the production of superoxide during phagocytosis. Inhibition of Rho by Tat-C3 exoenzyme (Tat-C3) blocked superoxide formation and curtailed the phagocytosis of ... More
Deregulation of collagen phagocytosis in aging human fibroblasts: effects of integrin expression and cell cycle.
AuthorsLee W, McCulloch CA
JournalExp Cell Res
PubMed ID9434634
Intracellular degradation of collagen by phagocytosis in fibroblasts is essential for physiological remodeling of the extracellular matrix in a wide variety of connective tissues but imbalances between degradation and synthesis can lead to loss of tissue collagen. As aging is associated with loss of dermal and periodontal collagen and with ... More