Validation of fluorescent-labeled microspheres for measurement of regional organ perfusion.
AuthorsGlenny RW, Bernard S, Brinkley M
JournalJ Appl Physiol
PubMed ID8335595
'Estimations of dog lung, pig heart, and pig kidney regional perfusion by use of fluorescent-labeled microspheres were compared with measurements obtained with standard radiolabeled microspheres. Pairs of radio- and fluorescent-labeled microspheres (15 microns diam, 6 colors) were injected into a central vein of a supine anesthetized dog and the left ... More
Fluorescent microspheres to measure organ perfusion: validation of a simplified sample processing technique.
A disadvantage of nonradioactive microsphere techniques is that the processing of samples is time-consuming and complex. We developed and validated a simplified processing method for the fluorescent microsphere (FM) technique. In seven anesthetized dogs with coronary artery stenosis up to six different FM and five different radioactivity labeled microspheres (RM) ... More