CR1-mediated ATP Release by Human Red Blood Cells Promotes CR1 Clustering and Modulates the Immune Transfer Process.
AuthorsMelhorn MI, Brodsky AS, Estanislau J, Khoory JA, Illigens B, Hamachi I, Kurishita Y, Fraser AD, Nicholson-Weller A, Dolmatova E, Duffy HS, Ghiran IC,
Journal
PubMed ID24022490
'Humans and other higher primates are unique among mammals in using complement receptor 1 (CR1, CD35) on red blood cells (RBC) to ligate complement-tagged inflammatory particles (immune complexes, apoptotic/necrotic debris, and microbes) in the circulation for quiet transport to the sinusoids of spleen and liver where resident macrophages remove the ... More
A simple method for monitoring changes in cell height using fluorescent microbeads and an Ussing-type chamber for the inverted microscope.
AuthorsCrowe WE, Wills NK
JournalPflugers Arch
PubMed ID1745609
'In this study, we report two developments for studies of ion transport in cultured epithelial cells. First, a convenient method is presented for measuring apparent cell height using fluorescent microbeads as high-contrast landmarks of the apical and basal cell surfaces. The apparent cell height is then used as an indicator ... More
Swelling-activated cation-selective channels in A6 epithelia are permeable to large cations.
AuthorsLi J, De Smet P, Jans D, Simaels J, Van Driessche W
JournalAm J Physiol
PubMed ID9688589
'Effects of basolateral monovalent cation replacements (Na+ by Li+, K+, Cs+, methylammonium, and guanidinium) on permeability to 86Rb of volume-sensitive cation channels (VSCC) in the basolateral membrane and on regulatory volume decrease (RVD), elicited by a hyposmotic shock, were studied in A6 epithelia in the absence of apical Na+ uptake. ... More
Transitional states of acrosomal exocytosis and proteolytic processing of the acrosomal matrix in guinea pig sperm.
In this study, we adapted a FluoSphere bead-binding assay to study the exposure and release of guinea pig sperm acrosomal components during the course of capacitation and acrosomal exocytosis. Prior to capacitation or the initiation of exocytosis, acrosomal proteins were not accessible to FluoSpheres coated with antibodies against two acrosomal ... More
An automatic monitoring system for epithelial cell height.
AuthorsVan Driessche W, De Smet P, Raskin G
JournalPflugers Arch
PubMed ID8272373
This paper describes an automatic method to measure cell height (h) of epithelia grown as monolayers on transparent filter supports. Tissues are mounted in an Ussing-type chamber enabling solution exchange on both sides. The apical and basal side of the epithelial cells are marked with fluorescent beads. The image of ... More
Hypotonicity activates a lanthanide-sensitive pathway for K+ release in A6 epithelia.
AuthorsDe Smet P, Li J, Van Driessche W
JournalAm J Physiol
PubMed ID9688850
The nature of the pathway for K+ release activated during regulatory volume decrease (RVD) in A6 epithelia was investigated by measuring cell thickness (Tc) as an index of cell volume and by probing K+ efflux with 86Rb as tracer for K+ (RRb). Cell swelling was induced by sudden reduction of ... More