'Aspirin is the most commonly used analgesic and antiinflammatory agent. In this study, at physiological concentrations, it profoundly inhibited CD40, CD80, CD86, and MHC class II expression on murine, GM-CSF + IL-4 stimulated, bone marrow-derived myeloid dendritic cells (DC). CD11c and MHC class I expression were unaffected. The inhibitory action ... More
Improved protocol for a microsphere-adhesion assay on living tissue slices.
AuthorsFörster E, Kaltschmidt C
JournalBiotechniques
PubMed ID10090988
Here, we describe a simple microsphere-adhesion assay to characterize adhesive cues on living tissue slices, which we use to study pattern formation in neural tissue. This assay was developed by modifying a cell-adhesion assay on living tissue slices. We replaced dissociated cells by fluorescent microspheres and then coated the microspheres ... More
Disialosyl galactosylgloboside as an adhesion molecule expressed on renal cell carcinoma and its relationship to metastatic potential.
AuthorsSatoh M, Handa K, Saito S, Tokuyama S, Ito A, Miyao N, Orikasa S, Hakomori S
JournalCancer Res
PubMed ID8620516
Aberrant glycosylation expressed in specific types of human cancer may define stage, direction, and fate of tumor progression. Well-studied examples are expression of sialosyl-Lewis(x) or sialosyl-Lewis(a) in colorectal carcinoma and histo-blood group A and H/Le(y) in lung cancer. In renal cell carcinoma (RCC), expression of sialosyl-Lewis(x) has no correlation with ... More