Fractal nature of regional ventilation distribution.
AuthorsAltemeier WA, McKinney S, Glenny RW
JournalJ Appl Physiol
PubMed ID10797111
'High-resolution measurements of pulmonary perfusion reveal substantial spatial heterogeneity that is fractally distributed. This observation led to the hypothesis that the vascular tree is the principal determinant of regional blood flow. Recent studies using aerosol deposition show similar ventilation heterogeneity that is closely correlated with perfusion. We hypothesize that ventilation ... More
Perfluorohexane vapor has only minor effects on spatial pulmonary blood flow distribution in isolated rabbit lungs.
AuthorsHübler M, Heller AR, Bleyl JU, de Abreu MG, Kroll T, Rössel T, Koch T
JournalAnesth Analg
PubMed ID15781532
'We tested the hypothesis that administration of perfluorohexane (PFH) vapor does not significantly affect the relative pulmonary blood flow (Qrel) distribution in isolated rabbit lungs. Fourteen isolated rabbit lungs were perfused with a Krebs-Henseleit buffer solution (flow 150 mL/min). Pulmonary afterload was set to 3 mm Hg. The lungs were ... More
Extending fluorescent microsphere methods for regional organ blood flow to 13 simultaneous colors.
AuthorsSchimmel C, Frazer D, Glenny RW
JournalAm J Physiol Heart Circ Physiol
PubMed ID11356604
'Seven fluorescent microsphere colors can be used in a single experiment to estimate regional blood flow without correcting for spillover of emitted fluorescence. To extend the method to 13 colors, we compared the accuracy of three methods for spillover correction. Fixed wavelength intensities were corrected by matrix inversion, and synchronous ... More
Validation of fluorescent-labeled microspheres for measurement of regional organ perfusion.
AuthorsGlenny RW, Bernard S, Brinkley M
JournalJ Appl Physiol
PubMed ID8335595
'Estimations of dog lung, pig heart, and pig kidney regional perfusion by use of fluorescent-labeled microspheres were compared with measurements obtained with standard radiolabeled microspheres. Pairs of radio- and fluorescent-labeled microspheres (15 microns diam, 6 colors) were injected into a central vein of a supine anesthetized dog and the left ... More
Fibroblast growth factor 4 induces vascular permeability, angiogenesis and arteriogenesis in a rabbit hindlimb ischemia model.
AuthorsRissanen TT, Markkanen JE, Arve K, Rutanen J, Kettunen MI, Vajanto I, Jauhiainen S, Cashion L, Gruchala M, Närvänen O, Taipale P, Kauppinen RA, Rubanyi GM, Ylä-Herttuala S
JournalFASEB J
PubMed ID12475908
'Previous studies have shown that fibroblast growth factor (FGF)-1, FGF-2, and FGF-5 induce therapeutic angiogenesis. Here, we investigated the potential of FGF-4 for therapeutic neovascularization in comparison to vascular endothelial growth factor (VEGF), using adenoviral gene transfer in a novel rabbit hind limb ischemia model, with ischemia restricted to the ... More
Development of a novel fluorescent microsphere technique to combine serial cerebral blood flow measurements with histology in the rat.
AuthorsDe Visscher G, Haseldonckx M, Flameng W, Borgers M, Reneman RS, van Rossem K
JournalJ Neurosci Methods
PubMed ID12573474
The aim of the study was to evaluate the microsphere technique for the quantitative assessment of regional cerebral blood flow (rCBF) at different time points in the same animal. Yellow-green and red fluorescent microspheres with a diameter of 15 microm were injected into the rat at two different time points ... More
Fluorescent microspheres to measure organ perfusion: validation of a simplified sample processing technique.
A disadvantage of nonradioactive microsphere techniques is that the processing of samples is time-consuming and complex. We developed and validated a simplified processing method for the fluorescent microsphere (FM) technique. In seven anesthetized dogs with coronary artery stenosis up to six different FM and five different radioactivity labeled microspheres (RM) ... More
Single-cell volume estimation by three-dimensional wide-field microscopy applied to astroglial primary cultures.
Astrocytes, which constitute a prominent part of the number and volume of brain cells, have a high capacity for controlling their volume, and astrocytic swelling is associated with a number of pathological states affecting the CNS. In order to understand the mechanisms for regulating cell volume in astrocytes better, it ... More
Characterization of retinal leukostasis and hemodynamics in insulin resistance and diabetes: role of oxidants and protein kinase-C activation.
AuthorsAbiko T, Abiko A, Clermont AC, Shoelson B, Horio N, Takahashi J, Adamis AP, King GL, Bursell SE
JournalDiabetes
PubMed ID12606527
Increases in leukostasis/monocyte adhesion to the capillary endothelium (leukostasis) and decreases in retinal blood flow may be causally associated and are implicated in the pathogenesis of diabetic retinopathy. In this study, we demonstrate that increases in leukostasis are observed in insulin-resistant states without diabetes, whereas decreases in retinal blood flow ... More
Segregation of saturated chain lipids in pulmonary surfactant films and bilayers.
AuthorsNag K, Pao JS, Harbottle RR, Possmayer F, Petersen NO, Bagatolli LA
JournalBiophys J
PubMed ID11916861
The physical properties of organized system (bilayers and monolayers at the air water interface) composed of bovine lipid extract surfactant (BLES) were studied using correlated experimental techniques. 6-Dodecanoyl-2-dimethylamino-naphthalene (LAURDAN)-labeled giant unilamelar vesicles (mean diameter approximately 30 microm) composed of BLES were observed at different temperatures using two-photon fluorescence microscopy. As ... More