IC Fixation Buffer
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IC Fixation Buffer

Intracellular fixation buffer for use in flow cytomerty, particularly for fixation in intracellular staining procedures. Composition is paraformaldehyde in phosphateRead more
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Catalog NumberQuantity
FB001100 mL
Catalog number FB001
Price (CNY)
1,194.00
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Ends: 31-Dec-2025
1,586.00
Save 392.00 (25%)
Each
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Quantity:
100 mL
Price (CNY)
1,194.00
Online Exclusive
Ends: 31-Dec-2025
1,586.00
Save 392.00 (25%)
Each
Add to cart

Intracellular fixation buffer for use in flow cytomerty, particularly for fixation in intracellular staining procedures. Composition is paraformaldehyde in phosphate buffered saline, pH 7.3.

These Made-to-Order catalog items have a 2 week lead time.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Cell Surface Antibodies, Intracellular Cytokine Antibodies
Quantity100 mL
Shipping ConditionWet Ice
Product TypeFixation Buffer
Solution TypeFixation Buffer
pH7.3
Unit SizeEach
Contents & Storage
Store at 2°C to 8°C. Do not freeze.

Frequently asked questions (FAQs)

What fixative should I use to fix my cells or tissues for antibody labeling?

Aldehyde-based fixatives (e.g., formaldehyde, glutaraldehyde) crosslink various cellular components, which helps retain proteins and cell morphology, but some antigens can be masked by the crosslinking, requiring antigen retrieval methods to unmask the antigen binding sites. Also, aldehyde-based fixation requires permeabilization to allow entry of antibodies. Organic solvents such as methanol and acetone condense proteins and permeabilize the cells, but cell morphology can be compromised. Surface antigens can be labeled without fixation.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.